Baorui Bio's nucleic acid extraction reagents (magnetic bead method) series products are now available!

Grand debut!

Nucleic acid extraction reagent kits (magnetic bead method) series products

Officially join the Baorui product family!

Guide

Molecular diagnosis, as one of the important fields of in vitro diagnosis, is currently a highly technical and promising sub-sector in the in vitro diagnosis industry. Nucleic acid extraction, as a crucial step in molecular diagnosis, its extraction quality is a key factor related to the accuracy of the test results. Nucleic acid extraction reagents (magnetic bead method) are a perfect combination of nanotechnology and biotechnology, and have many advantages over other extraction methods.The new series of nucleic acid extraction reagents (magnetic bead method) developed by Baorui Biotechnology has made a grand debut with their stable, sensitive and efficient performance, refreshing the product catalog!

Baorui Biological Nucleic Acid Extraction Reagent (Magnetic Bead Method)-- General Basic Edition

Baorui Biological Nucleic Acid Extraction Reagent (Magnetic Bead Method)- General Basic EditionIt is suitable for extracting high-quality and high-purity from liquid samples such as whole blood, plasma, serum, saliva, sampling swab wash fluid, and alveolar lavage fluidDNA and RNA. This reagent adopts a unique lysis buffer formula, which enables the sample to release nucleic acids under specific conditions such as salt ion concentration and pH value. Subsequently, based on the principle of nano-scale magnetic beads adsorbing nucleic acids, the extraction, enrichment and purification of nucleic acids are completed through the adsorption, transfer and release of magnetic beads by specially designed magnetic rods.

I. Product Advantages

-Stable, reliable and highly sensitive

★ The reagents are equipped with automated extraction devices, making the test results more stable and reliable.

★ High sensitivity: The extraction concentration of DNA virus nucleic acid can be as low as 10IU/ml, and that of RNA virus nucleic acid can be as low as 50IU/ml.

- Easy to operate and highly safe

★ Avoid centrifugation and manipulationFor convenience;

The automated extraction process only takes 17 minutes, and the purified nucleic acid can be directly used for downstream testing.

★ Free of toxic reagents such as chloroform and phenol, easy to handleSafer.

- Strong applicability and wide sample range -

★ Applicable to the extraction of various DNA and RNA nucleic acids;

It can easily extract nucleic acids from liquid samples such as oral/nasopharyngeal swab wash fluid, whole blood, plasma, serum, saliva, and alveolar lavage fluid.

Ii. Experimental Data

Pig blood samples were used as the test samples, and a certain competing product on the market was adopted respectively1. Nucleic acid extraction reagent (magnetic bead method), a certain marketed competing product 2. Nucleic acid extraction reagent (magnetic bead method), and Baorui Biological nucleic acid extraction reagent (magnetic bead method) were used to extract 200 μ l of nucleic acid samples. Three parallel samples were extracted from each, and the same amount of extracted products were used to determine the concentration and purity of nucleic acid. The results are as follows:

Product nucleic acid concentrationPurity determination statistics table

2. Dilute the pseudovirus sampleThe concentrations of RBCS-RNA were 10⁶ copies/ml, 10⁷copies/ml and 10⁸copies/ml respectively as the samples to be tested. 200 μ l of nucleic acid from the samples were extracted using a certain marketed competing product 1 nucleic acid extraction reagent (magnetic bead method) and the nucleic acid extraction reagent of Baorui Biology (magnetic bead method) respectively. Two parallel samples were extracted from each and detected using the high-sensitivity M5134 fluorescence quantitative RT-PCR reagent from Baorui. The results are as follows:

Product amplification resultCt value measurement statistics table

RBCS-RNA amplification curve graph

3. Dilute hepatitis B virusHBV positive plasma samples with concentrations of 10⁴, 10³, 10², and 10 IU/ml were selected as the test samples. 200 μ l of nucleic acid samples were extracted using a certain marketed competing product 3 nucleic acid extraction reagent (magnetic bead method) and Baorui Biological nucleic acid extraction reagent (magnetic bead method), and two parallel samples were extracted from each. And the detection was carried out using the high-sensitivity M2071 fluorescence quantitative PCR reagent from Baorui. The results are as follows:

Product amplification resultCt value statistics table

HBV-DNA amplification curve graph

4. Hepatitis B virus at different concentration gradientsHBV plasma was used as the sample to be tested. 200 μ l of DNA from the sample was extracted using the nucleic acid extraction reagent (magnetic bead method) from Baorui Biology, and then detected using the high-sensitivity M2071 fluorescence quantitative PCR reagent from Baorui. The results are as follows:

Product amplification resultCt valueStatistical table

HBV-DNA amplification curve graph

5. Hepatitis C virus at different concentration gradientsHCV plasma was used as the sample to be tested. RNA was extracted from 200 μ l of the sample using the nucleic acid extraction reagent of Borui Biotechnology (magnetic bead method), and the detection was carried out using the high-sensitivity M5134 fluorescence quantitative RT-PCR reagent of Borui. The results are as follows:

Product amplification resultCt valueStatistical table

HCV-RNA amplification curve graph

Conclusion

Baorui BiologyThe general basic version of nucleic acid extraction reagent (magnetic bead method) has excellent extraction performance. Compared with similar competing products, it offers a better extraction operation experience, faster speed and superior results. It can simultaneously meet the extraction requirements of nucleic acids from multiple types of liquid samples.

This kit, when used in conjunction with a nucleic acid extractor or workstation, not only simplifies the operation process and saves time, but also significantly increases the yield and purity of the extracted nucleic acids.