Baorui facilitates efficient influenza detection and builds a precise prevention and control network!

20-minute fully premixed rapid test, the new product from Baorui helps with efficient influenza detection and builds a precise prevention and control network!

Introduction

At the transition from winter to spring, the flu epidemic enters a high-incidence period in many places, and the workload of medical institutions for testing has increased significantly.

Experimental conditions

Test results of Tube B

ConclusionMD2104-X can remain stable for 7 days at 2-8℃ when in the primer-containing probe state. In subsequent experiments, only the template needs to be added to achieve efficient amplification, making the use of reagents more convenient.

Experimental conditions

Test methodThe buffer, enzyme and primer probe were mixed to prepare the working solution and subjected to six freeze-thaw treatments. A comparative test was conducted using the immediately prepared working solution of the same system as the control.

ConclusionMD2104-X can remain stable after six freeze-thaw cycles in the state containing primer probes.

Rapid amplification test✦

Experimental conditions

Test results of Tube A

Figure 1 shows the test results of amplification performance of RNA under the normal procedure

Figure 2 shows the test results of amplification performance under the rapid RNA program

Test results of Tube B

Figure 3 shows the test results of amplification performance of RNA under the normal procedure

Figure 4 shows the test results of amplification performance under the rapid RNA program

Test results of Tube C

Figure 5 shows the test results of amplification performance of RNA under the conventional procedure

Figure 6 shows the test results of amplification performance under the rapid RNA program

ConclusionThe amplification performance of the MD2104-X system shows that the Ct value difference between the rapid amplification program and the conventional amplification program is less than 1, indicating that this system is suitable for rapid PCR amplifiers or rapid programs for rapid amplification.

Anti-inhibition test✦

Experimental conditions

Test methodDilute the quality control samples of influenza A and B viruses respectively with TE Buffer, nucleic acid release agent and the nucleic acid release agent added to the oral swab to the concentration to be tested, and then directly add the sample, with an addition volume of 10μL for every 20μL

ConclusionThe amplification effect of MD2104-X on background samples of release agents and oral swabs is basically comparable to that of pure template amplification diluted with TE Buffer, and it has a strong anti-inhibitory effect on release agents and oral swabs.

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