One step to perfection! This no-extraction, customizable and freeze-drying fecal direct expansion reagent is definitely worth having

In recent years, with the development of molecular biology and the improvement of detection levels, many detection methods can directly conduct target detection from clinical fecal samples. Among them, qPCR detection has become an advanced routine diagnostic method in gastroenterology. On the one hand, it can be used for the detection of common pathogens (bacteria, viruses, parasites, etc.), and on the other hand, it can also be used for tumor screening Real-time detection of the expression levels of tumor-related proto-oncogenes and tumor suppressor genes, or monitoring of related epigenetic modifications such as methylation modifications.

Fecal sample processing: Take 0.2g of the fecal sample and add it to 1 mL of TE Buffer, thoroughly mix and liquefy. After instantaneous centrifugation, the supernatant was taken to obtain a 20% fecal sample. After dilution with sterile water, 0.5% and 1% fecal samples were obtained. The human total RNA template was diluted with fecal samples of the above two concentrations to obtain a human genomic template with a concentration of 4.64 pg/μL. Then, add it directly to the reaction system at a sample volume of 20% for amplification.

Note: In the above amplification diagrams, the amplification Ct values of the VIC and ROX channels increase with the increase of fecal sample addition, and the amplification Rn values increase with the increase of fecal sample additionwhileReduce.

Fecal sample processing: Take 0.2g of the fecal sample and add it to 1 mL of TE Buffer, thoroughly mix and liquefy. After instantaneous centrifugation, the supernatant was taken to obtain a 20% fecal sample. After dilution with sterile water, 0.5% and 1% fecal samples were obtained. Dilute the CA16 template with fecal samples of the above two concentrations; Then, add it directly to the reaction system at a sample volume of 20% for amplification.

Note: In the above amplification graph, for the product of Supplier M, as the fecal background increases, the Ct value increases and the Rn value decreases.

The Biori product, however, does not have its target amplification interfered with by the increase in fecal background, demonstrating a stronger anti-inhibitory effect.

Left Figure 1The freeze-dried form of the fecal direct expansion system;Figure 2 on the right: Morphology of the fecal direct expansion system after freeze-drying at 55℃ and accelerated for 30 days

Stool Direct RT Premix-UNG (Probe qRT-PCR) is a dedicated reagent specifically designed to bypass the nucleic acid extraction and purification steps of fecal samples and directly conduct fluorescence quantitative (probe method) RNA amplification detection of target genes. This product has a strong tolerance to inhibitors, which can simplify the testing process of fecal samples while maintaining the quality and integrity of the samples.

If you are engaged in single or multiple detections of RNA pathogens or diseased substances in fecal samples, and if you have the need for non-invasive, convenient and rapid fecal nucleic acid testing, welcome to try the Stool Direct RT Premix-UNG (Probe qRT-PCR) fecal direct expansion reagent of Baorui Biology.

Product NameStool Direct RT Premix-UNG (Probe qRT-PCR) 

Item Number: MD2124

Product Name (Freeze-dried) :Stool Direct RT Premix-UNG (Probe qRT-PCR) (DG) 

Item Number: FMD5124

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