amplification refractory mutation system (ARMS-PCR), also known AS allele specific PCR (AS-PCR).
The ARMS principle is based on the characteristic that Taq DNA polymerase lacks 3 '→5' extrane-correction activity. On the basis of allele-specific extension reactions, an extension reaction can only occur when the 3 'terminal base of the allele-specific primer is complementary to the base of the site to be detected, thereby distinguishing samples with point mutations from those with normal genotypes. The ARMS technology, which is used to detect known mutations, has been widely applied in individualized molecular detection of tumors, gene mutation and SNP locus detection, and has become one of the most important and advanced technologies for individualized molecular detection of tumors internationally. Its advantages in clinical application have been widely recognized in the industry.
Based on the principle of mutation arrest amplification, Baorui Biotechnology has developed Robustart ARMS Gene-typing premix-ung (Probe qPCR), a qPCR Premix for ARMS typing technology that takes into account both amplification performance and mismatch identification ability. It can effectively conduct molecular detection, gene mutation and SNP locus detection based on ARMS-PCR technology.
Product application
01Individualized molecular detection of tumors, gene mutation and SNP locus detection.
02Genetic disease screening, disease susceptibility gene detection。
03Breeding in agriculture and animal husbandry, inspection and quarantine of animals and plants。
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