As RNA ladders for measuring RNA size, the ones currently available on the market can be classified into two types based on molecular weight: Low Range and High Range. The molecular weight Range of the Low Range Ladder is usually between 100 and 1000bp, and it is suitable for analyzing small RNA molecules such as microRNA and snRNA. The molecular weight range of the High Range Ladder is usually between 200 and 6000bp, and it is suitable for analyzing larger RNA molecules. Such as mRNA, long non-coding RNA (lncRNA), viral RNA, etc。
With the continuous deepening of life science research and the rapid development of biotechnology, more and more biotechnology companies have begun to pay attention to the research and application of RNA. In 2023, Baorui Biotechnology has launched RNA Ladder 6000 with a size of 200-6000bp (Product number:) (BP-10-50), suitable for various RNA analysis experiments, such as RNA electrophoresis, RNA quantification, etc. High-range RNA ladders, as important reagents in RNA research and application, especially in fields such as gene therapy and biopharmaceuticals, are still in increasing demand.
Identification of RNA fragment sizeRNA Ladder provides a series of RNA fragments of known sizes. These fragments are arranged in sequence according to their molecular weight during electrophoresis and can be used as reference objects to determine the size of each fragment in the RNA sample to be tested.
This is particularly important in processes such as RNA extraction, purification, cloning, and gene expression analysis.
RNA quantitative predictionDue to RNA LadThe RNA concentration of each molecular weight standard band of derAll have been precisely measured, and thus can be used as references for RNA quantification.
By comparing the brightness of the RNA fragment to be tested with that of the Ladder, the amount of RNA to be tested can be roughly estimated.
Quality control of electrophoresis experimentsWhen conducting RNA electrophoresis experiments, RNA Ladder can help evaluate whether the experimental conditions are appropriate.
By observing whether the bands of the Ladder are clear and the resolution is good, further determine whether the quality of the gel, the concentration of the electrophoresis buffer and the pH value and other experimental conditions meet the requirements，
This helps ensure the accuracy and reliability of the experimental results.
When using RNA Ladder 12000, the following matters should be noted:
It is recommended to use newly prepared electrophoresis buffer, gel and appropriate electrophoresis conditions (voltage, time, gel concentration) to ensure the accuracy and reliability of the analysis results.
2. Select clean electrophoresis equipment and it is best to operate it separately from the DNA sample. Residual RNase in the DNA sample may affect the stability of RNA.
3. RNA is extremely sensitive to ribonuclease. To prevent RNA degradation, please wear protective gloves, use nuclease-free consumables for sample preparation, or treat the vessels with nuclease scavenger.
Baorui Biotech's new product, RNA Ladder 12000, helps you achieve more breakthroughs on your scientific research journey!
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