New Arrivals: "Zero Burden" Nucleic Acid extraction, Magnetic Bead Method nucleic acid Extraction Kit - Alcohol-free and no-Drying Series Now Available!

During the COVID-19 pandemic, "nucleic acid testing" broke through the circle for the first time and became well-known to the public. Nucleic acid extraction, as the "first step" and the most crucial one in nucleic acid testing, has seen a significant increase in market demand. However, most of the existing nucleic acid extraction reagents contain flammable and explosive alcohol substances, which impose strict storage conditions and are not conducive to long-distance transportation and daily laboratory storage. Moreover, in the case of large-scale sample testing, the usage frequency increases sharply. The evaporation of components such as ethanol or isopropanol also has certain adverse effects on the health of laboratory testing personnel. For instance, at high concentrations, it can slightly irritate the eyes and nose. Inhaling high-concentration vapors or ingesting them by mistake can cause symptoms such as headache, dizziness, depression, nausea, vomiting, paralysis, and coma.

The latest research and development by Baorui Biology

Nucleic acid extraction reagent (magnetic bead method) -Alcohol-free and non-air-drying series

Effectively address the above-mentioned health and transportation risks

Safer and more stable

Realize "zero-burden" nucleic acid extraction!

The Baorui Biological nucleic acid extraction Reagents (magnetic bead Method) - alcohol-free and non-drying series products include: Swab Quick Extract III (all components alcohol-free), Plasma Quick Extract Version (washing solution alcohol-free), and Whole Blood alcohol-free Version (washing solution alcohol-free). It is respectively applicable to swab sample washes from the mouth, nose and throat. Plasma/serum; High-quality and high-purity DNA and RNA were extracted from whole blood samples. This series of reagent kits employs magnetic beads with separation functions and a unique buffer system. Under specific conditions such as salt ion concentration and pH value, samples are lysed to release nucleic acids. Subsequently, based on the principle of nano-scale magnetic beads adsorbing nucleic acids, the extraction, enrichment and purification of nucleic acids are accomplished through the adsorption, transfer and release of magnetic beads by specially designed magnetic rods. It features good stability, simple operation, high efficiency and time-saving. The purified nucleic acid can be directly used for downstream detection.

Experimental Data 01

The RNA pseudovirus swab solution samples were diluted with non-inactivated sample preservation solution to concentrations of 200copies/mL, 500copies/mL, 10⁴copies/mL, and 10⁶copies/mL respectively as the samples to be tested, and the nucleic acid extraction reagent (magnetic bead method) of Baorui Biological was usedSwab quick Lift IIIExtract 200 μ l of nucleic acid samples, and extract 3 parallel samples from each sample. The extracted products were subjected to fluorescence quantitative RT-PCR amplification to detect the target nucleic acid, and the results are as follows:

Statistical table of Ct values for amplification results of RBCS-RNA products

RBCS-RNA amplification curve graph

Experimental Data 02

Dilute the RNA pseudovirus swab solution sample with non-inactivated sample preservation solution to a concentration of 10⁶copies/mL as the sample to be tested, and use the nucleic acid extraction reagent (magnetic bead method) from Borui BioSwab quick Lift IIIExtract 200 μ l of nucleic acid samples and collect 6 parallel samples within one day. The extracted products were subjected to fluorescence quantitative RT-PCR amplification to detect the target nucleic acid (duplicate well detection), and the relative standard deviation RSDr (or coefficient of variation CV) of the amplification Ct value results was calculated and statistically analyzed to obtain the repeatability precision. The results are as follows:

Statistical table of Ct values for amplification results of RBCS-RNA products

RBCS-RNA amplification curve graph

Experimental Data 03

The hepatitis B virus (HBV) plasma samples were diluted to concentration gradients of 10⁴IU/mL, 10³IU/mL, 100 IU/mL and 50 IU/mL respectively as the samples to be tested, and the nucleic acid extraction reagent of Baorui Biological (magnetic bead method) was usedPlasma quick extract versionExtract 200 μ l of nucleic acid samples and two parallel samples from each sample. The extracted products were subjected to fluorescence quantitative PCR amplification for the detection of target nucleic acids (duplicate well detection), and the results are as follows:

Statistical table of Ct value Determination of HBV-DNA product amplification results

HBV-DNA amplification curve graph

Experimental Data 04

The HBV-positive samples were diluted with whole pig blood as the sample background to concentrations of 10⁴IU/mL and 50IU/mL respectively as the test samples, and the nucleic acid extraction reagent (magnetic bead method) of Baorui Biology was usedWhole blood alcohol-free versionExtract 200 μ l of nucleic acid samples and two parallel samples from each sample. The extracted products were subjected to fluorescence quantitative PCR amplification for the detection of target nucleic acids (duplicate well detection), and the results are as follows:

Statistical table of Ct value Determination of HBV-DNA product amplification results

 HBV-DNA amplification curve graph

NoteBottledB）；96-well deep hole plate pre-encapsulated (W

Lift the swab quicklyIII：

Plasma Quick Extract version

Whole blood alcohol-free version