The basic principle of RNA electrophoresis is to achieve separation by taking advantage of the size and charge differences of RNA molecules. RNA molecules have a negative charge and can migrate towards the anode in an electric field. In agarose gels, RNA molecules of different lengths have different migration rates due to differences in size and structure. Among them, shorter RNA molecules migrate faster, while longer RNA molecules migrate more slowly. The separation of RNA molecules can be achieved by adjusting the pore size of agarose gel, electrophoresis conditions and electrophoresis time. Generally, molecular weight markers are used as references. Based on the migration distance of the reference and that of the RNA sample, the size of the RNA molecule can be determined. Under denatured conditions (such as using denatured gels), the migration rate of RNA has a linear relationship with the logarithm of molecular weight, so the molecular weight of RNA can be determined.
RNA Ladder is a molecular weight gradient composed of ssRNA fragments of different lengths. Through electrophoresis, it can show distinct bands and is a standard for identifying the size of single-stranded RNA molecules. Based on the known size of RNA fragments, RNA Ladder can help estimate the size of other RNA fragments. This is achieved based on the principle that the molecular weight is inversely proportional to the migration rate through the gel matrix. Therefore, RNA Ladder is indispensable in the study of RNA molecules. It can provide researchers with a reference for the size of RNA fragments, so as to better understand and analyze the structure and function of RNA molecules.
01. RNA size identification
RNA Ladder can be used as a standard for determining the size of single-stranded RNA during electrophoresis. By comparing the position of the bands, the molecular weight of the RNA to be tested can be inferred. This is of great significance for studying the structure, function and localization of RNA。
02. RNA quantification and purity analysis
The concentration and purity of the sample RNA can be estimated by comparing it with RNA markers of known concentrations. Meanwhile, RNA markers can also serve as internal references to evaluate the stability and repeatability of experimental results.
03. Transcriptional analysis
In transcriptional analysis, RNA markers can be used to detect the length and size of transcription products. The size and integrity of unknown transcripts can be determined by comparing them with RNA markers of known transcript sizes.
✔ Help achieve more breakthroughs in scientific research!
01
The new product of Baorui, RNA Ladder 6000, is composed of RNA molecules of different sizes that are mixed together after in vitro transcription of eight linear DNA templates. It includes eight reference bands of 200 bp, 500 bp, 1000 bp, 1500 bp, 2000 bp, 3000 bp, 4000 bp and 6000bp。
02
Compared with traditional RNA electrophoresis, the electrophoresis bands of RNA Ladder 6000 are clearer and have higher resolution. RNA fragments of different lengths can be clearly distinguished on the electrophoresis map, facilitating qualitative and quantitative analysis.
03
RNA Ladder 6000 adopts standardized operation procedures and is easy to use. This product is suitable for various electrophoresis conditions including non-denatured gels and denatured gels, and provides detailed user guides and standard operating procedures.
When using RNA Ladder, the following points should be noted:
3. RNA is extremely sensitive to ribonuclease. To prevent RNA degradation, please wear protective gloves, use nuclease-free consumables for sample preparation, or treat the vessels with nuclease scavenger.
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