If the extraction is fundamental, the purity will not be fundamental——Baorui High-Efficiency Plasmid Small Extraction Kit 

In molecular biology research, obtaining high-purity and high-quality plasmid DNA is the foundation for the success of downstream experiments such as cloning, transformation, and sequencing. Plasmid Mini Kit from Baorui Biotech, based on the classic principle of alkaline lysis and combined with efficient silica gel membrane adsorption technology, provides you with a rapid, stable and reliable plasmid extraction solution.

The Plasmid Mini Kit for plasmid extraction uses the alkaline lysis method to lyse cells. It utilizes a unique silica gel membrane adsorption column to specifically adsorb plasmid DNA under high salt and low pH conditions, removing proteins, RNA, genomes, and other impurities to obtain high-purity and high-quality plasmid DNA Each adsorption column can adsorb up to 30µg of plasmid DNA. It is suitable for the efficient extraction of plasmid DNA from 1-5 ml of overnight cultured Escherichia coli. The yield and quality of plasmid extraction are related to factors such as the type of host bacteria and culture conditions, cell lysis, plasmid copy number, plasmid stability, and the volume of bacterial liquid.

The plasmid DNA extracted by this kit can be directly applied to various routine molecular biology experiments such as enzyme digestion, ligation, PCR, sequencing and transformation.

｜Extraction principle｜

PyrolysisUnder alkaline conditions, SDS causes cell membranes to rupture, proteins to denature, and simultaneously releases plasmid DNA and chromosomal DNA.

"ZhongheAfter adding the neutralizing solution, the denatured chromosomal DNA forms an insoluble reticular precipitate with the protein-SDS complex, while the covalently closed circular plasmid DNA regains its natural conformation and exists in the supernatant.

PurificationThe supernatant was transferred to a silica gel membrane adsorption column. Under a specific salt concentration, the plasmid DNA was specifically adsorbed and impurities were washed away.

ElutionHigh-purity plasmid DNA can be obtained by elution with low-salt buffer or water. 

The extracted plasmidThe DNA OD260/OD280 ratio is stable at 1.8-2.0, with OD260/OD230> 2.0. There is no significant residue of protein or salt, and the purity meets the requirements of high-standard experiments。

As many as can be extracted30 µg of plasmid DNA。

The process has few steps, is easy to operate and saves time.The entire extraction process can be completed within 30 minutes。

The purified plasmids do not require further processing, and the extracted products can be directly used for transformation, enzymatic digestion, sequencing,PCR and other experiments。

Use BaoruiPlasmid Mini Kit and well-known competing products on the market were used to extract plasmids from the same amount of bacteria under the same conditions and eluted with 100 µL of eluent. The above results show that

lThe yield of plasmids extracted by Baorui products is comparable to or slightly better than that of its competitors.

lThe bands are clear and bright, noneRNA residue.

lBoth the OD260/OD280 and OD260/OD230 ratios meet the standards of high-quality plasmids.

 productionProduct information 

BaoruiPlasmid Mini Kit, with its stable performance, excellent purity and efficient process, has become a reliable choice for plasmid extraction in your laboratory.
Whether it's routine experiments or high-throughput preparations, it can give you a helping hand!