Amidst countless expectations, the nucleic acid extraction reagent (magnetic bead method) series products developed by Baorui Biotechnology have added a new member, also making a brilliant debut with their outstanding performance of stability, sensitivity and high efficiency.
New series of productsNucleic acid extraction reagent (Magnetic Bead Method) - General Quick Extraction VersionOn the basis of ensuring the accuracy and stability of the test results, reduce the time for nucleic acid purification.The automated equipment can extract 96 samples at one time, and the program time is only required10min! Boost rapid nucleic acid testing and intensify efforts on the front line of the fight against the epidemic!
Efficient extraction within 10 minutes
Stability and sensitivity are not limited to speed
This issue presents to you the new series of nucleic acid extraction reagents (magnetic bead method)
Come and have a look quickly
Nucleic acid extraction reagent (magnetic bead method)
General Quick Lift version
Baorui BiologyNucleic acid extraction reagent (Magnetic Bead Method) - General Quick Extraction VersionIt is suitable for extracting high-quality and high-purity DNA and RNA from liquid samples such as whole blood, plasma, serum, saliva, sampling swab wash fluid, and alveolar lavage fluid. This reagent employs magnetic beads with separation functions and a unique buffer system to lyze and release nucleic acids from samples under specific salt ion concentrations and pH values. Subsequently, based on the principle of nano-scale magnetic beads adsorbing nucleic acids, it uses specially designed magnetic rods to adsorb, transfer and release the magnetic beads, thereby completing the extraction, enrichment and purification of nucleic acids.
Hurry up!
Easy to operate, efficient and fastThe program for extracting 96 samples at one time by the automated equipment only takes 10 minutes，The purified nucleic acid can be directly used for downstream detection.
Strong applicabilityIt is applicable to the extraction of various DNA and RNA nucleic acids.
Extensive sample sizeIt can easily extract nucleic acids from liquid samples such as oral and nasopharyngeal swab wash fluid, whole blood, plasma, serum, saliva, and alveolar lavage fluid.
High sensitivityThe concentration of DNA virus nucleic acid extraction can be as low as10IU/mlThe concentration of RNA virus nucleic acid extraction is acceptableAs low as50IU/ml。
Steady!
Stable and reliableThe reagents are equipped with automated extraction devices, making the test results more stable and reliable.
Experimental data
1. Dilute the HCV-positive plasma samples to concentrations of 10⁴ IU/ml and 50 IU/ml respectively as the samples to be tested. Extract 200 μ l of nucleic acid from the samples using a certain marketed competing product 1 nucleic acid extraction reagent (magnetic bead method) and the nucleic acid extraction reagent from Baorui Biology (magnetic bead method) respectively, and extract 2 parallel samples from each. The extracted products were subjected to fluorescence quantitative RT-PCR amplification detection. The results are as follows:
Statistical table for Ct value determination of product amplification results
HCV-RNA amplification curve graph
2. Dilute the HBV-positive plasma samples to concentrations of 10⁴ IU/ml and 10 IU/ml respectively as the samples to be tested. Extract 200 μ l of nucleic acid from the samples using a certain marketed competing product 1 nucleic acid extraction reagent (magnetic bead method) and the nucleic acid extraction reagent from Baorui Biology (magnetic bead method) respectively, and extract 2 parallel samples from each. The extracted products were subjected to fluorescence quantitative PCR amplification detection. The results are as follows:
Statistical table for Ct value determination of product amplification results
HBV-DNA amplification curve graph
Iii. Dilute the RNA pseudovirus swab solution samples to concentrations of 1.4×10⁶copies/ml and 1.4×10³copies/ml respectively as the samples to be tested. Extract 200 μ l of nucleic acid from the samples using a certain marketed competing product 1 nucleic acid extraction reagent (magnetic bead method) and the nucleic acid extraction reagent from Baorui Biology (magnetic bead method) respectively. Extract two parallel samples from each. The extracted products were subjected to fluorescence quantitative RT-PCR amplification detection. The results are as follows:
Statistical table for Ct value determination of product amplification results
RBCS-RNA amplification curve graph
4. Use whole pig blood as the sample background to dilute the HBV-positive sample to a concentration of 10⁴ IU/ml as the sample to be tested. Extract 200 μ l of nucleic acid from a certain marketed competing product 2 nucleic acid extraction reagent (magnetic bead method) and the nucleic acid extraction reagent from Baorui Biology (magnetic bead method) respectively, and extract 2 parallel samples from each. The extracted products were subjected to the following tests respectively: ① Total nucleic acid concentration and purity detection; ② Fluorescence quantitative PCR amplification was used to detect HBV nucleic acid. The results are as follows:
Statistical table of nucleic acid concentration/Purity Determination of products
Statistical table for Ct value determination of product amplification results
HBV-DNA amplification curve graph
V. Pig whole blood was used as the sample to be tested. 200 μ l of nucleic acid samples were extracted respectively using a certain marketed competing product 1 nucleic acid extraction reagent (magnetic bead method), a certain marketed competing product 2 nucleic acid extraction kit (magnetic bead method), a certain marketed competing product 3 nucleic acid extraction kit (magnetic bead method), and the nucleic acid extraction reagent of Baorui Biology (magnetic bead method), with 2 parallel samples extracted from each. The extracted products were subjected to fluorescence quantitative PCR amplification detection (amplifying the nucleic acid fragments of endogenous porcine gene DNA). The results are as follows:
Statistical table for Ct value determination of product amplification results
MLN-DNA amplification curve graph
Conclusion
The extraction performance of Baorui Bio's nucleic acid extraction reagent (magnetic bead Method) Universal Quick Extraction version is excellent. Compared with similar competing products,The extraction operation offers a better experience, faster speed and superior results. It can simultaneously meet the extraction requirements of nucleic acids from multiple types of liquid samples and has strong market competitiveness。
This kit, when used in conjunction with a nucleic acid extractor or workstation, not only simplifies the operation process and saves time, but also significantly increases the yield and purity of the extracted nucleic acids.
Product details of nucleic acid Extraction Reagent (Magnetic Bead Method)
Specification | Item number |
bottleServing (32 people) | IME02-1-B32T |
Bottled (48 servings) | IME02-1-B48T |
Bottled (64 servings) | IME02-1-B64T |
Bottled (96 servings) | IME02-1-B96T |
| Pre-packed (32 servings) | IME02-1-W32T |
| Pre-packed (48 servings) | IME02-1-W48T |
| Pre-packed (64 servings) | IME02-1-W64T |
| Pre-packed (96 servings) | IME02-1-W96T |
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Zhuhai Baorui Biotechnology Co., Ltd. was established in 2012, specializing in the core raw materials field of nucleic acid diagnostic reagents. It is a unicorn seed enterprise in Zhuhai and a national high-tech enterprise.We have successfully developed multiple series of high-quality molecular diagnostic enzymes and their matching reagents, which have been recognized by many well-known domestic enterprises and research institutions and are widely used in the research and development and production of diagnostic reagents.Meanwhile, Baorui has made significant investments in mRNA vaccine raw materials, NGS library construction reagents, and digital PMultiple research projects such as CR amplification reagents and STR multiplex detection reagents have been conducted, and related new products have been successively launched on the market.
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