8.5 minutes! The new series of magnetic bead nucleic acid extraction reagents from Baorui Biology accelerate nucleic acid testing and continue to contribute to the fight against the epidemic!

Nucleic acid extraction reagent (magnetic bead method)

Swab quick lift version

Efficient, time-saving, stable and sensitive

Efficient and time-savingThe procedure for extracting swab samples by automated equipment only requires8.5min！

High sensitivityRNA virus nucleic acid extraction can be as low as20copies

Wide applicabilityApplicable to all kindsExtraction of DNA and RNA nucleic acids

Simple operationThe nucleic acid purification is completed in three steps: one-step lysis and binding, one-step cleaning, and one-step elution.

Stable and safeThe reagents are equipped with automated extraction devices, making the test results more stable and reliable.

It does not contain toxic reagents such as chloroform and phenol, making the operation safer.

The RNA pseudovirus swab solution samples were respectively diluted with non-inactivated sample preservation solution and inactivated sample preservation solution to concentrations of 10⁶copies/ml and 10³copies/ml as the test samples, and were adoptedBaorui Biological Nucleic Acid Extraction Reagent (Magnetic Bead Method) - Quick Swab Extraction VersionExtract 200 μ l of nucleic acid samples, and extract 4 parallel samples from each sample. The extracted products were subjected to: ① fluorescence quantitative RT-PCR amplification for the detection of pseudoviral nucleic acids; ② Fluorescence quantitative PCR amplification was used to detect endogenous gene fragments in humans. The results are as follows:

Statistical table of Ct values for amplification results of RBCS-RNA products

Figure 1. Amplification curve of RBCS-RNA

Figure 2. EF1a-DNA amplification curve graph

withInactivated sample preservation solutionDilute the RNA pseudovirus swab solution samples to concentrations of 10⁶copies/ml, 10⁴copies/ml, 10³copies/ml and 10²copies/ml respectively as the test samples and adoptBaorui Biological Nucleic Acid Extraction Reagent (Magnetic Bead Method) - Quick Swab Extraction VersionExtract 200 μ l of nucleic acid samples, and extract 6 parallel samples from each sample. The extracted products were subjected to fluorescence quantitative RT-PCR amplification for the detection of pseudoviral nucleic acids. The results are as follows:

Statistical table of Ct values for amplification results of RBCS-RNA products

Figure 3. Amplification curve of RBCS-RNA

Dilute the RNA pseudovirus swab solution samples with non-inactivated sample preservation solution to concentrations of 10 respectively⁶copies/ml、10⁴copies/ml、10³copies/ml and 10²copies/ml were used as the sample to be tested, andBaorui Biological Nucleic Acid Extraction Reagent (Magnetic Bead Method) - Quick Swab Extraction VersionExtract 200 μ l of nucleic acid samples, and extract 6 parallel samples from each sample. The extracted products were subjected to fluorescence quantitative RT-PCR amplification for the detection of pseudoviral nucleic acids. The results are as follows:

Statistical table of Ct values for amplification results of RBCS-RNA products

Figure 4. Amplification curve of RBCS-RNA

Baorui Biological Nucleic Acid Extraction Reagent (Magnetic Bead Method) - Quick Swab Extraction VersionIt features good stability, simple operation, high efficiency and time-savingThe purified nucleic acid can be directly used for downstream detection.

Product details of nucleic acid extraction Reagent (magnetic Bead Method)