Messenger RNA (mRNA), as an intermediate for information transmission, has been widely used in the past few years. Theoretically, mRNA can produce any protein on demand within a patient's cells, thereby achieving the treatment of diseases. However, the application of linear mRNA faces many challenges due to its inherent defect of being easily degradable.
More and more studies have found that circRNA formed by reverse splicing to create a covalent closed circular structure protects circRNA from degradation by exonucases and is more stable compared to mRNA. In addition, circRNA has advantages that mRNA cannot replace, such as low immunogenicity and long-lasting expression, which have drawn more researchers' attention to it.
Preparation of circRNA by enzyme-linked method
Intron self-splicing for circRNA preparation
No matter which preparation process is adopted, linear RNA is always the precursor of circRNA. Eliminating the interference of linear RNA is the key to obtaining high-purity circRNA. circRNA tolerates RNase R digestion. High-quality RNase R can completely digest linear RNA without harming circRNA, achieving the goal of enriching circRNA.
Baorui Biotech has developed an RNase R product with high purity, high activity and strong specificity, providing support for the next generation of RNA vaccine drugs.
The high-efficiency RNase R (Ribonuclease R) of Baorui Biotechnology is a ribonuclease exonuclease derived from the RNR superfamily of Escherichia coli and dependent on Mg²⁺, which completely hydrolyzes linear RNA into dinucleotides and trinucleotides from the 3'→5' direction. It is not sensitive to circular RNA and double-stranded RNA and can be used in the production of special structure Rnas, such as circular RNA (circRNA), lasso structure RNA (lariat RNA), double-stranded RNA with a 3' protruding end of less than 7 nucleotides, and tRNA with complex structures, etc. RNase R is often used in gene expression and alternative splicing studies. It can digest linear RNA to enrich circ RNA or lariat RNA.
Remove linear RNA from circ RNA and apply it to the identification, enrichment and purification of circ RNA
Research on Variable Shear
Identify intron lasso structure RNA
Research on Gene Expression
01 Protein purity (SDS-PAGE) ≥99%
02 Baorui Biotech's highly efficient RNase R specifically digests linear RNA
The high-efficiency RNase R of Baorui Biotechnology was added to the reaction system containing linear RNA for reaction. The results showed that when the RNase R enzyme digestion reaction time was only 10 minutes,
The linear RNA bands have disappeared, indicating that RNase R can rapidly and efficiently digest linear RNA.
03 Competitive Analysis of High-Efficiency RNase R from Baorui Biotechnology
Enzyme digestion tests were conducted on the products before and after 1μg ring formation using equal amounts of the high-efficiency RNase R from Baorui Biotechnology and two domestic and foreign competing products, J1/J2. The results show that, compared with competing products, the high-efficiency RNase R of Baorui Biotechnology has stronger specificity, is more specific to linear RNA, and has almost no effect on circRNA, while the amount of circRNA of competing companies has changed after enzymatic digestion. Secondly, with the same amount of enzyme, the high-efficiency RNase R from Baorui Biotechnology can fully enzymatize linear RNA, while competitors still have residues.
The multiple freeze-thaw cycles of the high-efficiency RNase R from Baorui Biotechnology do not affect the efficiency and specificity of enzymatic digestion
The high-efficiency RNase R of Baorui Biotechnology can be repeatedly frozen and thawed up to 20 times without affecting the enzymatic digestion efficiency and specificity.
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