As the "throat link" of sequencing experiments, the quality of DNA purification directly determines the output of downstream data, and choosing the right magnetic beads is the key to breaking the deadlock! Today, we bring you a must-have product in the sequencing field - high-throughput sequencing-grade DNA purification magnetic beads. We provide a comprehensive analysis from principle to application, helping you take the first step in your experiment firmly!
The confidence of high-quality magnetic beads lies in their precise structure.
Biori ® NGS DNA Clean Beads adopts a three-layer design of "core - magnetic layer - functional layer", perfectly meeting sequencing-level requirements:
Using ultra-pure polystyrene as the base material, the particle size uniformity once reached over 95%, avoiding adsorption differences caused by uneven particle size and ensuring consistent purification efficiency for each sample tube.
Wrapped with high-purity iron (III) oxide, the magnetic response speed is less than 3 seconds. It settles rapidly without residue during magnetic attraction. When combined with an automated platform, it can complete the processing of 96-well samples within 40 minutes.
The surface carboxyl modification density is precisely controlled at 50-60 μmol/g, and it specifically binds to DNA through the "ion bridge" mechanism, effectively reducing the non-specific adsorption rate.
The core principle of SPRI: Dual buffs of efficient purification and intelligent sorting
The product is based on the classic SPRI (Solid-phase Reversible Immobilization) technology and innovatively optimizes the buffer system to achieve an integrated "purification + sorting" process: under the synergistic effect of high salt and PEG, the DNA hydration layer is disrupted, and the phosphate groups form stable binding with the carboxyl groups of the magnetic beads through sodium ions. The low-salt environment during elution can quickly break the "ion bridge", and the DNA recovery rate is as high as over 95%.
What is even more worth mentioning is that by adjusting the amount of magnetic beads used, fragments can be precisely sorted - low-proportion magnetic beads enrich high-molecular-weight DNA over 5Kb for PacBio/Nanopore sequencing, while high-proportion magnetic beads recover short fragments for NGS library construction. One bottle of magnetic beads meets the needs of multiple scenarios.
ForFor high-frequency issues in sequencing laboratories, Baorui Biotechnology offers systematic solutions from magnetic bead design to process control:
Residual inhibitors cause PCR amplification failure → Solution: Special sealing process of the functional layer significantly reduces the adsorption of proteins and polysaccharides. After purification, the DNA OD260/280 is stabilized at 1.8-2.0, which can be directly used for library construction without interference.
Low recovery rate of special samples such as FFPE → Solution: Optimize the hydrophilic modification on the surface of magnetic beads, design a high adsorption mode for degraded samples, and increase the recovery rate by more than 30% compared with ordinary magnetic beads.
Long waiting time for magnetic bead clarification: Reduce the diameter of the magnetic beads, optimize the magnetic bead Buffer, and accelerate the flow rate of the magnetic beads while ensuring their full suspension. Conventional library amplification products can be clarified immediately after incubation.
Advantage One
The same product was purified with different ratios of magnetic beads, and the product recovery rate was excellent.
AdvantageTwo
The magnetic bead loading capacity is high.
AdvantageThree
The distribution of the recovered products from fragment sorting is highly consistent with that of XP magnetic beads.
AdvantageFour
Flexibly recycle fragments of different lengths according to demand.
A good experiment begins with good materials! Scan the QR code to apply for the trial pack and let this "little magnetic bead" become a "big guarantee" for your sequencing data!
