In the current era when molecular detection technology is deeply integrated into large-scale screening scenarios, the operation isConvenience"TestStabilityAnd the accuracy of the results has already become the core demand of molecular diagnosis. Traditional qPCR testing requires manual and cumbersome preparation of components such as buffers, enzymes, primers, and probes. This not only makes the process lengthy and imposes strict requirements on the operational skills of personnel, but also often leads to problems such as missed addition, incorrect addition, and sample addition deviation due to human negligence, resulting in the failure to deliver the test results on schedule. Traditional qPCR can no longer meet the strict standards of large-scale testing.
To accurately break through the deadlock and directly address the pain points of the industry, Baorui Biotech has launched an iterative version with great forceDNA single-tube fully premixed reagent (including all components)。This reagent optimizes the core components of the reaction in advance and premixes them with the probes of downstream enterprises. During detection, only the sample needs to be added to quickly complete the construction of the reaction system. Reduce the risk of human error from the root, enhance the repeatability of experiments and the stability of detection, comprehensively improve the efficiency of detection, and help downstream enterprises achieve in large-scale screeningFaster, simpler and more reliableThe detection application.
High sensitivity
High-sensitivity amplification suitable for low-concentration DNA templates
Good stability
Primers, probes and reaction solution mixes (including buffer, enzyme system and Mg ion) can be fully premixed and stored at room temperature for 3 months.
Customer-friendly
When reagent manufacturers use it, they only need to add the probe and template, and it can be used. At the same time, this reagent is used to develop single-tube premixed detection reagents, which simplifies production, makes operation convenient, and complements the automation platform perfectly.
Figure 1 Amplification diagram of sensitivity detection
Table 1Statistics of sensitivity detection data
ConclusionThe reagent has high sensitivity and can achieve full detection at 5 ps/T in both bacterial and respiratory four-level detection systems.
Figure 2 Amplification diagram of single-tube fully premixed stability detection at 50℃ for 8 days
Table 2: If the ct value change is less than 1, it is evaluated as accelerated stability; otherwise, it is unstable. A change in the Rn value less than 10% is rated as stable; otherwise, it is rated as unstable.
ConclusionThe performance of the Baorui Bio M2331 DNA quadruple priming probe, fully premixed and accelerated at 50℃ for 8 days, remains basically stable.
Figure 3 shows the amplification diagram of a single-tube fully premixed 1M detection in the respiratory tract model
Table 3 Statistics of single-tube fully premixed 1M detection data in the respiratory tract model
ConclusionThe performance of the Baorui Bio M2331 with a quadruple priming probe for the respiratory tract, fully premixed, remains basically stable for one month at 2-8℃ and room temperature.
Figure 4 shows the amplification diagram of a single-tube fully premixed 1M detection in the bacterial model
Table 4 Statistics of detection data of single-tube fully premixed 1M in the bacterial model
ConclusionThe performance of the Baorui Biological M2331 quadruple priming probe with bacteria, fully premixed, remains basically stable for one month at 2-8℃ and room temperature.
Figure 5 Amplification diagram of the room-temperature 3M single-tube fully premixed stability test of the African swine fever model
Table 5 Statistics of Stability Test Data of 3M single tube fully premixed at room temperature
ConclusionThe performance of Baorui Bio M2331, which is fully premixed with dual probing for African swine fever, remains basically stable after being placed at room temperature for three months.
Figure 6 Amplification diagram of single-tube fully premixed stability test at 2-8℃ and 12M
ConclusionThe performance of Baorui Bio M2331, which is fully premixed with double probing for African swine fever, remains basically stable after being stored at 2-8℃ for one year.
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