Are you still struggling with DNA extraction from plant samples?
Grinding, centrifugation, and purification are carried out layer by layer, which is time-consuming and laborious. Moreover, polysaccharide and polyphenol samples are prone to inhibitory amplification. After working hard for a long time, you still can't get the ideal result?
Don't let the cumbersome steps slow down the pace of the experiment any longer!
The BR1A503 Plant Tissue Direct Hifi PCR Kit(Dye plus) is here!
Innovate the plant PCR experimental process, bid farewell to the cumbersome steps of DNA extraction, and directly add samples for amplification, doubling the efficiency of plant molecular experiments and making the results more accurate!
—— Plant Tissue Direct Hifi PCR Kit (Dye plus) ——
The core highlight buffs are fully stacked
Solve many pain points of plant PCR
No DNA extraction required
Samples can be directly added to plant leaves, pulp, stems and roots
Optimized for polysaccharide and polyphenol plants
The PCR system is constructed in 30 minutes, with an ultra-fast PCR extension rate.
Suitable for all kinds of common plants and complex samplesIt is also compatible with direct expansion of fungi, blood and cells.
The fidelity of the amplifying enzyme of Plant Hifi Master Mix in the component reagents is approximately 154 times that of the common Taq enzyme, significantly reducing the mutation rate.
Adapt to multi-scenario experiments
A great helper for plant molecular research
Multi-dimensional performance verification
The strength is visible.
Take about 3×3mm ² of Arabidopsis thaliana leaves and add 100μL of Plant Lysis Buffer for simple lysis treatment. Then, take 1μL of the supernatant as a template to amplify 300bp, 600bp, 900bp and 2000bp of the target genes respectively for rapid PCR amplification, with an extension rate of 2s/kb.
The results show thatBR1A503 can normally amplify target genes of different sizes in Arabidopsis thaliana.
All sample types are covered
It can handle complex samples as well
After simple Lysis treatment with Plant Lysis Buffer on shiitake mushrooms (fungi), strawberry pulp (polysaccharide plants), spinach (polyphenol plants), rice leaves and corn cobs, 2μL of the supernatant was taken as the template; 2μL of yeast liquid and human blood were directly taken for PCR.
The results show thatBR1A503 can not only perform rapid PCR direct expansion on common plant leaves, but also on fungal plants, polysaccharide plants, polyphenol plants, bacteria and blood.
Different tissues of the same plant
The amplification effects are all excellent
Fresh spinach leaves, stems and roots were taken for rapid PCR direct amplification, and the results all showed normal amplification.
High fidelity definitely outperforms its peers
The result is more accurate.
The most commonly used standard Taq DNA polymerase is derived from aquatic Thermophilic bacteria. It lacks 3'→5' exonuctase correction activity, which will introduce more mutation errors during the PCR process, thereby affecting the judgment of correct results.
The high-fidelity enzyme of Hifi Master Mix in BR1A503 has a fidelity approximately 154 times that of the common Taq enzyme. The following figure shows the amplification fidelity of polymerases from different companies determined by the second-generation sequencing method.
The results show thatThe fidelity of Hifi Master Mix is superior to that of well-known brands at home and abroad.
Product Information
