Bst 2.0 HS

Bst DNA Polymerase is derived from Bacillus stearothermophilus DNA PolymeraseⅠ. Through genetic engineering technology, the 5' →3' DNA polymerase activity was retained, while the 5' →3' exonuctase activity was removed. It has strand displacement ability and can be used in DNA strand displacement reactions and LAMP (Loop-mediated isothermal amplification) amplification, etc. Bst 2.0HS is a hot-start constant-temperature amplidase obtained by reversibly modifying the genetically modified Bst 2.0 DNA polymerase. It can completely block the enzyme's activity at room temperature, thus enabling the establishment of reactions at room temperature, preventing non-specific amplification, and enhancing reaction efficiency. In addition, Bst2.0HS does not require separate activationSteps.

1．8 U/µL Bst 2.0 HS 

2．10×LAMP Basic Buffer (Mg²+free (Optional)

3．250 mM MgSO₄(Optional

*10×LAMP Basic Buffer (Mg²+ free) does not contain dNTP and Mg²+. Please add dNTPs and MgSO₄ when preparing the reaction system.

1. For LAMP

2. Aptam-modified hot-start isothermal amplifiers do not require a separate activation step.

3. Establish the reaction at room temperature to prevent non-specific amplification and improve the reaction efficiency.

lamp isothermal amplification related technologies