Bst 3.0 HS

Bst DNA Polymerase 来源于 Bacillus stearothermophilus DNA PolymeraseⅠThrough genetic engineering technology, the 5' →3' DNA polymerase activity was retained, while the 5' →3' exonuclease activity was removed. It has strand displacement ability and can be used in DNA strand displacement reactions and LAMP (Loop-mediated isothermal amplification) amplification, etc.
Bst 3.0HS is a hot-start constant-temperature amplifying enzyme obtained by reversibly modifying the genetically modified Bst 3.0 DNA polymerase. It can completely block the enzyme's activity at room temperature, thus enabling the establishment of reactions at room temperature, preventing non-specific amplification, and enhancing reaction efficiency. In addition, Bst3.0HS does not require a separate activation step.

1．8 U/µL Bst 2.0 HS 

2．10×LAMP Basic Buffer (Mg²+ free)（选配） 

3.250 mM MgSO₄ (optional)

*10×LAMP Basic Buffer (Mg²+ free) does not contain dNTP and Mg²+. Please add dNTPs and MgSO₄ when preparing the reaction system.

1. It can be used for LAMP, aptam-modified hot-start isothermal amplifiers, without the need for a separate activation step.

2. Strong anti-inhibition ability, high impurity tolerance, and high sample compatibility.

3. An anti-pollution system can be established at room temperature to effectively prevent aerosol contamination of the products.

1. lamp isothermal amplification related technologies.

2. Establishment of a pollution prevention system.