Colorimetric RT Lamp Kit (RY) (Bst2.0 HS)

LAMP (Loop-mediated isothermal amplification) loop-mediated isothermal amplification reaction is a nucleic acid amplification technology. This kit uses visual constant-temperature amplification and can be used for qualitative detection of DNA in samples (such as viruses). Bst2.0 is derived from Bacillus stearothermophilus DNA Polymerase. Compared with the large fragment of wild-type Bst DNA polymerase, this enzyme can effectively increase the amplification speed, yield, etc.
Bst2.0 possesses 5 '→3' DNA polymerase activity and strong strand displacement ability, but lacks 3 '→5' exonuclease activity. It can be used in DNA strand displacement reactions and LAMP amplification, etc. Bst2.0HS is a hot-start isothermal polymerase obtained by using reversible modification technology on the basis of Bst2.0. It can completely block the enzyme's activity at room temperature, establish reactions at room temperature, prevent non-specific amplification, and improve reaction efficiency.
In addition, Bst2.0HS DNA polymerase does not require a separate activation step.

1. Bst2.0 HS (8 U/μL)

2. Neoscript RTase (200 U/μL)

3. 2×RT-LAMP Premix Buffer Ⅱ

The LAMP visual detection method suitable for RNA samples allows for visual observation of the results without the need to detect fluorescence.

2. Establish the reaction at room temperature to prevent non-specific amplification and improve the reaction efficiency.

3. Based on pH indication, the Wells are purplish red before the reaction. After the reaction, the positive Wells turn yellow and the negative Wells remain purplish red.