Neoscript® RTase Ⅱ

Neoscript RTase II is a highly active reverse transcriptase obtained through targeted modification based on the Baorui Neoscript RTase through a rational screening platform. It features higher activity, temperature tolerance, and more prominent inhibitor tolerance, making it suitable for high-temperature reverse transcription and rapid reverse transcription. It is conducive to eliminating the adverse effects of RNA advanced structure and non-specific factors on cDNA synthesis, and has higher stability and reverse transcription synthesis ability.

1．200 U/µL Neoscript RTase Ⅱ

2.5 ×First-Strand Buffer (optional)

The 5×First-Strand Buffer does not contain dNTPs. Please add DNTPS when preparing the reaction system.

The temperature range for reverse transcription is 42-55℃.

2. It has excellent specificity and sensitivity.

3. High activity, suitable for all kinds of rapid and highly sensitive detection.

4. It has strong tolerance to inhibitors from sources such as blood and swabs, as well as residual substances like phenols and alcohols from sample pretreatment.

1.cDNA synthesis and detection of various RNA pathogens.

2. Gene expression analysis.

3. Rapid amplification, direct amplification, and nucleic acid testing POCT applications.

1. Inhibition resistance of different RT enzymes

Experimental conditions

Experimental results

Definition: The larger the IC50 value of an enzyme inhibitor, the stronger the enzyme's anti-inhibitory ability against that inhibitor.
Summary: E42 reverse transcriptase has good anti-inhibitory performance.

2. Comparative testing of clinical samples

Experimental conditions

Experimental results

Note: The same detection system MD2104 was used, with only the reverse transcriptase replaced, and the enzyme amount remained consistent.
Summary: In the rapid direct amplification scenario, the E42 reverse transcriptase of Baorui Biotech effectively amplifies throat swab samples, and its inhibitory resistance is superior to that of E13.

3. High-temperature resistance test of E42

Experimental conditions

Experimental results

Test method: Prepare E42 into the MD2104-000817 system and test different reverse transcription temperatures.

Ct value statistics

Summary: The E42 reverse transcriptase from Baorui Biotechnology is compatible with reverse transcription at a temperature of 60℃.