AcuGenix™ Plant Tissue Direct Hifi PCR Kit

AcuGenix™ Plant Tissue Direct High-Fidelity PCR Kit

High-fidelity amplification without nucleic acid extraction is adapted for difficult-to-process plant samples

This kit is a dedicated solution specifically designed for direct high-fidelity PCR amplification of plant tissues. It does not require pre-extraction of nucleic acids and can directly amplify using untreated plant tissues as templates. It is compatible with various plant sample types such as leaves, stem segments, roots, seeds, and callus, with excellent repeatability between batches. The kit is equipped with a proprietary lysis buffer system, which can rapidly lyse various plant materials within minutes and release complete genomic DNA. There is no need to pre-remove proteins, RNA or secondary metabolites such as polysaccharides and polyphenols. The crude lysate can be directly used for PCR amplification, greatly simplifying the experimental process.

1. Minimalist process without extraction:
It eliminates the cumbersome nucleic acid extraction steps, saving over 70% of the operation time compared to the conventional process. At the same time, it reduces the risk of aerosol contamination caused by opening the lid and avoids the loss of templates due to the purification step.

2. Ultra-high fidelity performance
The 2× premix solution loaded with engineered antibody-blocked hot-start DNA polymerase has been verified by standardized mismatch rate detection, and its fidelity is approximately 154 times that of wild-type Taq DNA polymerase. This enzyme possesses excellent DNA binding affinity and rapid and continuous synthesis capabilities. It still shows outstanding compatibility with templates containing complex secondary structures or partial degradation, meeting the amplification requirements of difficult-to-lyze plant tissues. It can significantly reduce the sequence error rate in downstream cloning, sequencing, and genome editing verification.

3. Samples that are difficult to adapt to inhibitors:
The premix has been optimized through a proprietary formula, with the addition of extension enhancers and specific accelerators. It can effectively overcome the interference of common PCR inhibitors such as polysaccharides and polyphenols in plant tissues, and still achieve stable amplification of difficult-to-process samples that are hard to amplify with conventional direct PCR kits.

4. Easy operation and stable batch:
All core components (hot-start high-fidelity DNA polymerase, dNTP, and optimized buffer) have been pre-mixed into a 2× system. Only primers and templates need to be added to initiate the reaction, reducing pipetting errors and enhancing experimental repeatability.

1. Crop genotyping (SNP, SSR, InDel analysis).

2. Detection of genetically modified crop incidents.

3. Molecular marker development (CAPS, dCAPS, SCAR marker verification).

4. Germplasm resource identification and diversity analysis.

5. Validation of plant genome editing results (CRISPR-Cas9 knock-in/knockout validation).

6. Sanger sequencing of plant targets/Preparation of targeted NGS library templates.