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RIPA(Radio Immunoprecipitation Assay) Lysis Buffer is a traditional rapid lysis buffer for cells and tissues, mainly used for extracting soluble proteins from animal cells and tissues. The protein samples obtained from its lysis can be used in conventional experiments such as Western Blot, IP and Elisa.

There are many formulations for RIPA lysis buffer, which can be roughly classified into strong, medium and weak categories based on their lysis strength.

Samples such as cell or tissue extracts contain many endogenous proteases, phosphatases, etc., which can easily lead to protein degradation or demodification in the extracts, thereby affecting subsequent protein detection. Therefore, adding appropriate inhibitors of proteases, phosphatases, etc. to extracts and other samples is an effective method to prevent protein degradation and demodification.

The core components of Baorui Biological protease and phosphatase inhibitors are as follows:

The Bradford protein concentration determination method is one of the commonly used and highly sensitive protein concentration determination methods at present.

The principle is that Coomassie Brilliant Blue G-250 combines with the basic and aromatic amino acids of proteins to form a blue compound. The maximum absorbance wavelength of this substance is 595 nm. The absorbance value has a good linear relationship with the protein content within a certain concentration range. By measuring the absorbance value and comparing it with the absorbance value of standard proteins, the protein concentration can be calculated So as to achieve rapid determination of protein concentration.

This kit is based on the principle that protein molecules can dissolve Cu in alkaline solutions^{2 +}Restore to Cu⁺2,2 '-biquinoline-4,4' -dicarboxylic acid (BCA) and Cu⁺The combination forms a purple complex. Within a certain range, the depth of its color is directly proportional to the protein concentration. The protein standard solution is used as the standard curve, and the protein content in the test sample is determined by colorimetry.

Tris-Glycine-SDS Running Buffer is commonly used as the electrophoresis buffer for protein SDS-PAGE. This buffer solution is a 10-fold concentrated solution. Before use, it only needs to be diluted to 1× working solution with deionized water.

This product is a denatured reduced protein loading buffer。

This product is suitable for the TrIS-glycine electrophoresis system. It adopts a premixed formula of upper and lower layers of gel. Just add the improved coagulant and it will gel, which is simple and fast. The upper layer of tape provided is blue/red, and the sampling holes are clear and easily distinguishable, making it convenient for sampling. Blue/red concentrated gels can be used to distinguish gels containing different samples. This product comes with an improved accelerator, which has better stability and catalytic efficiency. No additional TEMED needs to be added during the glue mixing process.

The 180 kDa Prestained Protein Marker is composed of 10 proteins of different molecular weights pre-stained in three colors, with molecular weights ranging from 13 to 180 kDa. Among them, 75 kDa is an orange-red band, 13 kDa, 17 kDa, 36 kDa, 45 kDa, 60 kDa, 100 kDa, 140 kDa and 180 kDa are blue bands, and 25 kDa is a green band. This product is used in SDS-PAGE experiments, which can clearly indicate the electrophoresis process and accurately determine the molecular weight of the target protein. In Western blot experiments, it can be used to monitor the transfer effect of PVDF membranes or NC membranes and determine the detection status of target proteins. This product can be applied to different buffer systems of Tris-Glycine and Bis-Tris gels (Tris-Glycine, MOPS and MES). The protein bands of this product have all been calibrated by non-pre-stained protein markers, and the band sizes are accurate.

The 10× Fast Transfer Buffer is a highly efficient, safe and non-toxic rapid transfer buffer specially designed for Western Blot wet rapid transfer. This product is a 10 times concentrated buffer and needs to be diluted to a 1× concentration with an appropriate amount of deionized water and ethanol before use.

PVDF membrane, namely polyvinylidene fluoride membrane, is a solid-phase support widely used in Western blotting. This membrane is hydrophobic, and its pore sizes vary. As the pore sizes gradually decrease, its binding ability to low-molecular-weight proteins becomes increasingly firm。

NC Membrane, also known as nitrocellulose membrane, is the most commonly used blotting membrane for protein and nucleic acid hybridization. The NC membrane is hydrophilic and does not need to be activated in methanol or ethanol. It only requires simple wetting in water during transfer. Its uniform pore structure can bind biomolecules and is mainly used in Western blot to transfer proteins from various gel matrices to membranes。

10×TBST Buffer is a widely used isotropic buffer salt solution in biological experiments. It is mainly used in Western Blot experiments to wash away non-specifically bound antibodies and other reagents on the membrane, thereby reducing the background and enhancing the signal-to-noise ratio.

This product is suitable for Immunofluorescence (IF), Immunohistochemistry (IHC) and Immunocytochemistry. In experiments such as IC), the preparation of blocking solutions, the dilution of primary or secondary antibodies, and the washing after incubation。

DAB Peroxidase Substrate Kit is a HRP-DAB substrate chromogenic reagent kit product developed by Baorui Biotechnology.

This product contains all the reagents for detecting horseradish peroxidase (HRP). Staining of PVDF membranes and NC membranes suitable for Western Blot hybridization. DAB (3, 3’n-diaminobenzidine Tertrahydrochloride) is a commonly used substrate for horseradish peroxidase (HRP). Under the catalysis of horseradish peroxidase, DAB will produce a brown precipitate that is insoluble in water and ethanol. HRP fixed by immune reaction can catalyze the transformation of the substrate DAB at the location of the target protein into a brown compound. The location and expression of the target protein can be determined based on the color reaction。