UNG (Uracil-DNA Glycosylase)

Uracil-dna Glycosylase (UNG or UDG for short) is derived from the recombinant clone expression of Escherichia coli, with a molecular weight of 25 kDa. It can catalyze the release of free Uracil from single-stranded and double-stranded DNA containing uracil, and has no activity against RNA. It can be applied to prevent contamination caused by PCR amplification products.
The principle of its action is based on the fact that if dUTP is used to replace dTTP and incorporated into DNA during the PCR reaction, forming a PCR amplification product containing dU bases, this enzyme can selectively break the glycosidic bonds of the U bases in single-stranded and double-stranded DNA, thereby degrading the PCR amplification product.

UNG enzyme 1U/μL

Digest the dUTP template at 50℃; Inactivation by heating at 95℃。

Pollution prevention and control