Tth DNA Polymerase (Item No. E06)

Tth DNA Polymerase is a thermally stable DNA polymerase derived from thermophilic bacteria Thermus thermophilus HB8, which, in the presence of Mg²+, can catalyze the 5 'to 3' direction DNA template-dependent deoxynucleotide polymerization. It can be widely applied to various PCR reactions. Compared with Taq enzyme, this product is more tolerant to inhibitory components such as blood-derived inhibitors. In the presence of Mn²+, it exhibits stronger reverse transcription activity and can be applied to single-tube one-step RT-PCR reactions. The Tth DNA Polymerase developed by our company is a high-purity Tth DNA Polymerase product obtained through recombinant expression of Escherichia coli and a multi-step chromatography process.

Item No. : E06

Specification5U/μL

It has 5 '-3' polymerase and 5 '-3' exonuclease activities; No 3 '-5' exonuclease activity; The 3 'end of the PCR product is A.

Reagent composition

1．5 U/µL Tth DNA Polymerase 

2．10×Tth RT Buffer Basic (Mn²+ free)（选配） 

3.25mM Mn(OAc)₂ (optional)

*10×Tth RT Buffer Basic (Mn²+ free) does not contain DNNTP and Mn²+. Please add dNTPs and Mn(OAc)₂ when preparing the reaction system.

Product features

It has a higher reverse transcription temperature, which can effectively address the issue of reduced reverse transcription efficiency caused by the complex secondary structure of RNA. And it helps to improve the specificity of hybridization between primers and templates.

2. The single-enzyme one-step RT-PCR amplification reaction is adopted, which makes the system simpler and the cost lower.

3. Tth is derived from thermophilic bacteria and has higher stability than the traditional two-enzyme one-step RT-PCR system.

4. Be capable of establishing an RT-PCR anti-contamination reaction system using UNG.

5. Tth has a higher tolerance to inhibitors than Taq enzyme.

Scope of application

1. Applicable to qPCR

2. Applicable to qRT-PCR

3. It can be matched with an anti-pollution system

PCR/qPCRRT-PCR(qPCR) (requires Mn²+)

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