Taq DNA Polymerase(Item No. E02)

Taq DNA Polymerase is a highly thermally stable DNA polymerase derived from thermophilic bacteria Thermus aquaticus. This enzyme reaction requires the participation of Mg²+ and can catalyze the polymerization of deoxynucleotides dependent on DNA templates in the 5 'to 3' direction. The Taq DNA Polymerase developed by our company is a high-purity enzyme protein obtained by recombinant expression of the Thermus aquaticus DNA Polymerase gene in Escherichia coli and through multi-step chromatography separation and purification. It has the same properties as natural Taq DNA Polymerase.

Item No. : E02

Specification5 U/µL

It has 5 '-3' polymerase and 5 '-3' exonuclease activities; It has no 3 '-5' exonuclease activity and no calibration function.

Reagent composition

1．5 U/µL Taq DNA Polymerase 

2．10×PCR Buffer Ⅱ (Mg²+free (Optional)

3.25mM MgCl₂ (optional)

*10×PCR Buffer Ⅱ (Mg²+free of dNTP and Mg²+When preparing the reaction system, please add dNTPs and MgCl₂ for use.

Product features

At 70 to 75℃, the elongation rate is 1 to 2 kb per minute.

2. The 3 'end of the PCR product is A, and the product can be directly cloned into a T vector.

3. Applicable to common PCR and RT-PCQualitative and quantitative detection of R.

4. It is applicable to fluorescence quantitative PCR detection, DNA sequencing, etc.

It is applicable to qualitative and quantitative detection by common PCR and RT-PCR.

2. It is applicable to fluorescence quantitative PCR detection, DNA sequencing, etc.

3. There is a freeze-drying version available.

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