2×Fast Direct Premix-UNG (Probe qPCR)

Fast Direct Premix-UNG (Probe qPCR) is a dedicated reagent specifically designed to bypass the nucleic acid extraction and purification steps of the sample and directly conduct fluorescence quantitative (probe method) DNA amplification detection of the target gene. It contains DNA polymerase that has been genetically modified and screened for rapid amplification. The PCR reaction can be completed within 30 minutes.

This product has a strong tolerance to inhibitors and can be used for direct DNA amplification detection of anticoagulated whole blood, plasma, serum, throat swabs and other samples without DNA extraction and purification. In addition, this reagent adopts an optimized formula of qPCR dedicated Buffer and UNG/dUTP anti-contamination system, which can effectively prevent false positive amplification caused by residual PCR products and aerosol contamination.

2×Fast Direct Premix-UNG (Probe qPCR)
This reagent already contains hot start enzyme, UNG, PCR Buffer and MgCl₂Ingredients such as dNTPs and stabilizers.

Antibody modification, 95℃, hot start for 1 to 5 minutes.

2. It supports the mixing of primers, probes and reagents in one tube to maintain good stability.

3. It has excellent tolerance to inhibitors from sample sources such as blood, swabs, and tissue homogenates. The direct sample addition ratio is ≤5% for whole blood and ≤30% for plasma and serum.

Detection of DNA viruses related to humans and animals.

2. POCT application scenarios.