Lamp Premix (Probe) (Bst2.0 HS)

LAMP (Loop-mediated isothermal amplification) loop-mediated isothermal amplification reaction is a nucleic acid amplification technology. This kit is a constant temperature amplification using the probe method (RNase HⅡ) and can be used to detect DNA in samples (such as viruses).

Bst 2.0 DNA polymerase has 5 '→3' polymerase activity and strong strand displacement activity, but no 5 '→3' exonuclease activity. Compared with wild-type Bst DNA polymerase and large fragments, this enzyme can effectively increase amplification speed, yield, etc. Bst 2.0HS is a hot-start isothermal polymerase obtained by using reversible modification technology based on Bst 2.0 DNA polymerase. It can completely block the enzyme's activity at room temperature, establish reactions at room temperature, prevent non-specific amplification, and improve reaction efficiency. In addition, Bst 2.0HS DNA polymerase does not require a separate activation step.

1. Freeze-thaw stability verification of HW205-P01

Experimental conditions

Experimental results

NTC detection standard: NTC initiation time >30 minutes

Summary: The HW205-P01 system has good stability after 15 freeze-thaw cycles. Under the human-based detection system, the difference in CT values between low, medium and high concentration templates after 15 freeze-thaw cycles is less than 2 minutes.

2 HW205-P01 Accelerated Stability test

Experimental conditions

Experimental results

NTC detection standard :NTC initiation time >30 minutes



Summary: The HW205-P01 system has good stability after 14 days of acceleration. Under the human-based detection system, the difference in CT values after 14 days of acceleration under low, medium and high concentration templates is less than 2 minutes.