2×RT-LAMP Premix (Probe)

LAMP (Loop-mediated isothermal amplification) loop-mediated isothermal amplification reaction is a nucleic acid amplification technology. This kit is a constant temperature amplification using the dye method (Eva Green) and can be used for qualitative detection of RNA in samples (such as viruses). Bst2.0 DNA Polymerase is derived from Bacillus stearothermophilus DNA polymerase I. Compared with wild-type Bst DNA polymerase and large fragments, this enzyme can effectively increase amplification speed, yield, etc. Bst2.0 DNA polymerase possesses 5 '→3' DNA polymerase activity and strong strand displacement ability, but lacks 5 '→3' exonuclease activity. It can be used for DNA strand displacement reactions and LAMP (Loop-mediated isothermal amplification) amplification, etc. Bst 2.0HS is a hot-start isothermal polymerase obtained by using reversible modification technology based on Bst 2.0 DNA polymerase. It can completely block the enzyme's activity at room temperature, establish reactions at room temperature, prevent non-specific amplification, and improve reaction efficiency. In addition, Bst 2.0HS DNA polymerase does not require a separate activation step.

This kit employs Lamp technology, designing 4 to 6 specific LAMP-specific primers and RNase HⅡ -dependent probe primers for the target sequence. It utilizes the chain displacement of Bst isothermal amplifying enzyme and the activity of polymerase to amplify the target fragment.RNase HⅡ specifically recognizes the DNA double strands bound to the probe and the target sequence, and cuts the probe primers to emit fluorescence signals. The presence of amplification is determined by detecting the fluorescence signals with an instrument.

Item No. : HW206-P01

Freeze-dried item number: FHW1008-P01

Reagent composition

2×RT-LAMP Premix Buffer Ⅱ、Bst2.0 HS (8U/µL)、NeoscriptRTase (200U/µL)、RNase HⅡ (50mU/µL)

 

Recommended application：

1.optimumwithinDetection of RNA samples by LAMP probe methodFluorescence is detected by instruments。

2.Establish the reaction at room temperature to prevent non-specificityAmplification to enhance reaction efficiency.

3. It can be freeze-dried in full components.

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