DNaseⅠ

Deoxyribonuclease I (abbreviated as DNase I) is a deoxyribonuclease I obtained through recombinant expression of Pichia coli strains. This enzyme can efficiently digest single-stranded or double-stranded DNA fragments. The activity of DNase Ⅰ is highly dependent on Ca2+ levels and can be measured by Mg²+Or Mn²+Activate. At Mg²+Under the existing conditionsDNase Ⅰ can randomly cut DNA fragments from any site. In Mn²+Under the existing conditions, DNase I can perform cleavage at approximately the same sites of double-stranded DNA.Form flat-end DNA fragments or sticky ends containing 1-2 nucleotide protrusions. When the working pH range is between 7 and 8, DNase Ⅰ has the best endonuclease activityIt can be applied to remove single or double strands from various RNA samplesDNA fragment.

The activity of DNase Ⅰ is highly dependent on Ca^{2 +}It is at a certain level and can be Mg^{2 +}Or Mn^{2 +}Activate. At Mg^{2 +}Under the existing conditions

2.DNase Ⅰ can randomly cut DNA fragments from any site. In Mn^{2 +}Under the existing conditions, DNase I can perform cleavage at approximately the same sites of double-stranded DNA.

3. Form flat-end DNA fragments or sticky ends containing 1-2 nucleotide protrusions.

It can be applied to remove single-stranded or double-stranded DNA fragments from various RNA samples.