Deoxyribonuclease I (abbreviated as DNase I) is a deoxyribonuclease I obtained through recombinant expression of Pichia coli strains. This enzyme can efficiently digest single-stranded or double-stranded DNA fragments. The activity of DNase Ⅰ is highly dependent on Ca2+ levels and can be measured by Mg²+Or Mn²+Activate. At Mg²+Under the existing conditionsDNase Ⅰ can randomly cut DNA fragments from any site. In Mn²+Under the existing conditions, DNase I can perform cleavage at approximately the same sites of double-stranded DNA.Form flat-end DNA fragments or sticky ends containing 1-2 nucleotide protrusions. When the working pH range is between 7 and 8, DNase Ⅰ has the best endonuclease activityIt can be applied to remove single or double strands from various RNA samplesDNA fragment.
Product item Number: E204
Reagent composition：
1. 2U/μL DNase Ⅰ
2. 10×DNase Ⅰ Reaction Buffer（选配）
Product features
The activity of DNase Ⅰ is highly dependent on Ca²+It is at a certain level and can be Mg²++ or Mn²+Activate. At Mg²+Under the existing conditionsDNase Ⅰ can randomly cut DNA fragments from any site. In Mn²+Under the existing conditions, DNase I can perform cleavage at approximately the same sites of double-stranded DNA.Form flat-end DNA fragments or sticky ends containing 1-2 nucleotide protrusions.
Recommended applications
It can be applied to remove single-stranded or double-stranded DNA fragments from various RNA samples.
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