HPV18 E6/E7 Residual DNA Fragment Analysis and Detection Kit (PCR-Fluorescence Probe Method)

This kit employs the PCR fluorescence probe method. Primer probes are designed for the conserved sequences of the HPV18 E6/E7 genome, and are used in conjunction with ready-to-use calibrators for the quantitative detection of residual E6/E7 DNA fragments in biopharmaceutical samples. This kit, when used in conjunction with the nucleic acid extraction or purification kit from Zhuhai Baorui, can detect the residual amount of E6/E7 DNA fragments in as fast as 2 hours.

Linear range: The linear range of all four fragments is 5copies/μL-1E+07copies/μL, amplification efficiency: 95%-105%, R²> 0.99.

Figure 1 Linear plot of the E6-134 fragment

Figure 2 Linear plot of fragment E6-204

Figure 3 Linear plot of segment E7-115

Figure 4 Linear plot of the E7-202 fragment

2. Calibration sample amplification diagram

Figure 5 Amplification diagram of the calibration sample of the E6-134 fragment

Figure 6 Amplification diagram of the calibration sample of the E6-204 fragment

Figure 7 Amplification diagram of the calibration sample of the E7-115 fragment

Figure 8 Amplification diagram of the calibration sample of the E7-202 fragment

3. Product performance summaryThe above data is for reference only. For specific product performance indicators, please refer to the performance verification report of the corresponding item number.