HEK293 Residual DNA Fragment Analysis and Detection Kit (PCR-Fluorescence Probe Method)

Human cell line HEK293 is a very common expression vector in cell therapy, gene therapy and biopharmaceuticals, and is widely usedrawProtein production, vaccines, anti-cancer reagentsAnd recombinant adenovirus coating, etc.

The "Technical Guidelines for the Study and Evaluation of In Vivo Gene Therapy Products (Trial)" states that corresponding control strategies should be formulated based on the assessment results.rawChan Ruo shiwithSwollenTumor cell lines (such as Hela cells), tumorigenic cell lines, or cells carrying tumorigenic genes or virus-derived sequences (such as HEK 293T cells) must be ensured to be intactWhile living cells remain, the residual amount of DNA and residual fragments also need to be examinedbigsmallCarry out control and reasonably set standard limits. If possible, it is recommended to minimize the residue as much as possibleThe DNA should be controlled within 10ng per dose, and the residual DNA fragments should be largesmallKeep it below 200bp.This kit employs the PCR fluorescence probe method. Primer probes are designed for the conserved sequences of the HEK293 genome to quantitatively detect residual HEK293 DNA fragments in samples"Segment quantity.

This kit, when used in conjunction with the Baorui Biological nucleic Acid extraction or Purification Kit, can detect HEK293 DNA in as little as 2 hourssliceResidual amount of the segment.

This kit has undergone comprehensive methodological validation in accordance with relevant regulations, guidelines and other document requirements, and can provide performance validation reports.

Product item Number: BP-QN60-100

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