Universal DNA Purification Kit

The Universal DNA Purification Kit, a universal DNA purification kit, utilizes the specific adsorption of DNA by nucleic acid purification columns to recover DNA fragments from TAE and TBE agarose gels. It can also be directly used to purify DNA fragments from crude DNA products obtained by other methods, such as PCR products or enzymatic digestion products Remove impurities such as other organic compounds, proteins, inorganic salt ions, and oligonucleotide primers. The DNA purified by this kit can be directly applied to various operations such as enzymatic digestion, ligation, cloning and sequencing.

1. Fast and convenient: The recycling work can be completed in just over ten minutes.

2. Color indication: Can be indicated by fingerinThe color change of the reagent can be used to determine whether it is suitable for column centrifugation.

3. Wide range of purified fragments: DNA fragments ranging from 100 to 10kb can be recovered.

4. High recovery rate: Recovery ratehighUp to 80%.

5. Wide application: It is suitable for the recovery of TAE or TBE agarose gels, as well aswithRecovery of PCR amplification products.

The purified DNA can be directly applied to molecular biology experiments such as enzyme digestion, ligation, cloning and sequencing.

1. Purification recovery efficiency and product quality test results

 figure1BaoruiBR2E301And the purification and recovery of competing products of different lengthsDNAFragment result

The direct purification and recovery of 100 bp to 10 kb fragments from PCR or enzymatic digestion products using Borui BR2E301 all demonstrated high recovery efficiency and good band integrity, meeting the requirements of various downstream applications.

2. Gel recovery efficiency and product quality test results

Figure 2 shows the results of recovering DNA fragments of different lengths using Borui BR2E301 and competing gels

The DNA fragments of 100 bp, 250 bp, 2.5 kb, 4 kb and 10 kb were respectively cut and recovered. The results show that this kit has a good recovery efficiency for DNA fragments of different lengths. The recovered products are complete and pure, and the effect is superior to that of similar competing products.