2×FastAmpli Premix-UNG Ⅳ (Probe qPCR) (020305)

FastAmpli Premix-UNG IV (Probe qPCR) (020305) is a special reagent for qualitative and quantitative reactions in real-time PCR using the probe method, containing DNA polymerase that can be rapidly amplified through genetic modification and screening. The PCR reaction can be completed within 30 minutes. This product has been optimized with a Buffer system and is suitable for multiple amplifications. This reagent uses a mixed enzyme of anti-inhibitory amplifying enzyme and UNG enzyme, as well as an optimized Buffer system containing dUTP. It not only enables good amplification of the target gene in samples containing inhibitors, but also effectively prevents false positive reactions caused by residual PCR products and aerosol contamination. This product is compatible with fluorescence quantitative PCR instruments from most manufacturers, such as Applied Biosystems, Eppendorf, Bio-Rad and Roche, etc.

2×FastAmpli Premix-UNG IV (Probe qPCR)（020305）

This reagent already contains hot start enzyme, UNG, PCR Buffer and MgCl₂Ingredients such as dNTPs and stabilizers

The amplification enzyme was modified with double antibodies to block the DNA polymerization activity and the 5-3 exonuclitic active site, and was thermally started at 95℃ for 30 seconds to 1 minute.

2. It has strong tolerance to inhibitors from sources such as blood and swabs, as well as residual substances like phenols and alcohols from sample pretreatment.

3. It has strong applicability to various types of tests.