CUT&Tag Assay Kit for Illumina

CThe UT&Tag Assay Kit for Illumina is a library construction kit developed for the Illumina high-throughput sequencing platform for studying protein-DNA interactions. CUT&Tag (Cleavage Under Targets and Tagmentation) is a novel technique for studying protein-DNA interactions: After chromatin proteins are in situ bound by specific antibodies, targeted binding and precise cleavage using Protein A-Tn5 fusion protein can effectively generate fragment libraries with high resolution and extremely low background.

Compared with the traditional ChIP-Seq, the CUT&Tag method provided by this kit has the advantages of low cell usage, simple operation, short cycle and high signal-to-noise ratio. It is suitable for detecting specific transcription factor binding sites or the occurrence sites of specific histone modifications, etc., and can be applied in research fields such as epigenetics, early embryonic development, stem cells, and tumors.

1. Low initial sample size: Even as low as 100 cells can yield highly specific and high signal-to-noise ratio data.

2. pA-Tn5 evolution: A rationally designed mutant enzyme with extremely high transpostion efficiency, enhancing resolution at low cell volumes.

3. Optimization of extraction module: High-quality extraction magnetic beads, efficient recovery of 200bp small fragments, compatible with accurate fragment sorting processes.

4. Buffer system optimization: Promote efficient and highly specific binding of antibodies and transposases, and reduce background noise.


1.Application scenarios

Identification of transcription factor binding sites.

② Analysis of histone modifications.

③ Research on chromatin openness and construction of gene regulatory networks.

④ Single cells and CUT&Tag

⑤ Epigenetic regulation of organoids

2. Application type

① Basic research: Chromatin dynamics, transcriptional regulation, epigenetic mechanisms.

② Disease research: Cancer heterogeneity, screening for markers of neurodegenerative diseases.

③ Drug development: Verification of the mechanism of action of targeted epigenetic drugs.

3. Main application fields

① Academic research (70%) : Universities, research institutions.

② Pharmaceutical enterprises (25%) : Discovery of tumor drug targets.

③ Clinical diagnosis (5%) : Early exploration stage

1. Experimental procedure

2. Quality control data:

Conclusion: Under the same cell input (10,000 cells), the BR data were all superior to Supplier Y.

Conclusion: Under different cell input amounts, the BR 50bp small fragment (corresponding to the 200bp library) was more than Supplier Y.

3. Bioinformatics data

Conclusion: Under different cell input amounts, BR showed significant enrichment of TSS/TES, and the effect was no less than that of Supplier Y. At the minimum input of 100 cells, the enrichment of TSS/TES remained significant.

Hattori T, et al. Antigen clasping by two antigen-binding sites of an exceptionally specific antibody for histone methylation. Proc. Natl. Acad. Sci. U.S.A. 2016

PMID: 26862167

Fong N, et al. RNA Pol II Dynamics Modulate Co-transcriptional Chromatin Modification, CTD Phosphorylation, and Transcriptional Direction. Mol. Cell 2017

PMID: 28506463