AcuGenix™ Magnetic Sputum Nucleic Acid Extraction Kit

AcuGenix™ Magnetic Bead Method Sputum Nucleic Acid Extraction Kit

Specialized Solution for Co-extraction of Respiratory pathogen nucleic acids: Overcoming the Challenge of extracting High-viscosity sputum and Stably detecting trace amounts of Mycobacterium tuberculosis.

This kit is a dedicated solution for nucleic acid extraction based on the magnetic bead method, specially designed for respiratory sputum samples. In response to the three major extraction pain points of sputum samples, namely high mucin content, high host cell background, and complex PCR inhibitors, the self-developed silanoly-functionalized superparamagnetic microspheres and pre-optimized buffer system are adopted, which can efficiently lyse the mucin matrix, digest host cells, and rapidly release DNA from pathogen sources.

1. Specialized optimization for phlegm
Customized development for sputum samples with high viscosity and high inhibitor content eliminates the need for additional mucin digestive enzymes or cumbersome pretreatment, addressing the pain points of low sputum extraction yield and high inhibitor residue in general extraction kits.

2. Safe and non-toxic, easy to operate
No phenol-chloroform extraction, ethanol precipitation or column crossing operation is required. The entire process is completed in a closed tube, significantly reducing the risk of aerosol contamination and meeting the operational requirements of BSL-2 biosafety laboratories.

3. Highly sensitive recovery of trace pathogens
It still maintains a stable recovery efficiency for trace respiratory pathogens as low as 10 copies per reaction and can efficiently capture refractory Mycobacterium tuberculosis.

4. High purity ensures detection reliability
After strict batch quality control, the nucleic acid yield is stable and the purity is excellent (A260/A280 ratio 1.8~2.0), which can efficiently remove PCR inhibitory factors in mucin, host genomic DNA and respiratory secretions, avoiding false negatives in downstream tests.

5. Downstream full-scenario compatibility
The extracted nucleic acids do not require additional purification and can be directly used in the AcuGenix™ series of NGS library construction reagents without optimizing the reaction system.

Pathogen unbiased identification (mNGS) of lower respiratory tract infections.

2. Research and development of multiple respiratory pathogen detection panels and clinical research.

3. Analysis of drug resistance genotypes of refractory pathogens such as Mycobacterium tuberculosis.

4. Host-pathogen interaction study of sputum samples.

5. Development of clinical molecular diagnostic methods for respiratory tract infections.