Robustart Premix OT-UNG is a new type of highly stable single-tube reaction premix reagent developed by our company. It can achieve long-term stable storage at -20℃ under the condition that primers and probes are premixed into single tubes, thus making the preparation and use of PCR reaction systems more convenient and increasing the consistency of test results in different batches. This product can effectively inhibit the decline in amplification performance caused by non-specific annealing of primers or primer polymerization at room temperature, enhance the specificity and stability of the reaction, and make the detection of low-concentration templates more stable. This product adopts an optimized formula of qPCR dedicated Buffer and UNG/dUTP anti-contamination system, which can obtain a good standard curve within a wide quantitative range and effectively prevent false positives caused by contamination of PCR products. This reagent is compatible with fluorescence quantitative PCR instruments from most manufacturers, such as Applied Biosystems, Eppendorf, Bio-Rad and Roche, etc.
Item No. : M421
Reagent composition4×Robustart Premix OT（Probe qPCR）
Product features
Robustart Premix OT adopts a new type of hot start enzyme and can achieve rapid hot start within 2 to 5 minutes.
2. This product features excellent system stability. After being premixed into single-tube reagents with primers and probes, it can maintain long-term stable performance under storage conditions of -20℃.
3. This product features higher specificity and sensitivity, making it suitable for use as a high-sensitivity fluorescence quantitative PCR detection reagent.
Scope of applicationIt can be combined with an anti-pollution system
Experimental example
37℃ accelerated stability test
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