RT Lamp Premix (Dye Ⅱ) (Bst2.0 HS)

LAMP (Loop-mediated isothermal amplification) loop-mediated isothermal amplification reaction is a nucleic acid amplification technology. This kit is a constant-temperature amplification using the dye method and can be used for qualitative detection of DNA in samples such as viruses. Bst2.0 is derived from Bacillus stearothermophilus DNA Polymerase. Compared with the large fragment of wild-type Bst DNA polymerase, this enzyme can effectively increase the amplification speed, yield, etc. Bst2.0 possesses 5 '→3' DNA polymerase activity and strong strand displacement ability, but lacks 3 '→5' exonuclease activity. It can be used in DNA strand displacement reactions and LAMP amplification, etc.

Bst2.0HS is a hot-start isothermal polymerase obtained by using reversible modification technology on the basis of Bst2.0. It can completely block the enzyme's activity at room temperature, establish reactions at room temperature, prevent non-specific amplification, and improve reaction efficiency. In addition, Bst2.0HS DNA polymerase does not require a separate activation step

1. Bst2.0 HS (8 U/μL)

2. Neoscript RTase (200 U/μL)

3. 2×RT-LAMP Premix Buffer Ⅱ

4. 50×RT-LAMP Fluorescent Dye Ⅱ

The LAMP dye method for RNA sample detection requires an instrument to detect fluorescence.

2. Establish the reaction at room temperature to prevent non-specific amplification and improve the reaction efficiency.

3. It can be freeze-dried in full components.