T7 RNA polymerase 3.0

The by-product dsRNA generated during the IVT reaction strongly stimulates RNA receptors within cells, triggering innate immune signals. BaoruirawThe T7 RNA Polymerase 3.0 independently developed and multi-stage purified has high transcriptional catalytic activity for different templates and different nucleic acid types. Compared with the common T7 RNA Polymerase, the content of the by-product dsRNA produced by transcription of T7 RNA Polymerase 3.0 was significantly reduced. T7 RNA Polymerase 3.0 can precisely recognize the T7 promoter region (5'-TAATACGACTCACTATAG-3') and, starting from G in this region, transcribed the subsequent DNA sequence into single-stranded RNA, using natural nucleotides or modified nucleotides as substrates. Through short-term in vitro transcription incubation, under an appropriate reaction buffer system, users can efficiently obtain a large amount of RNA products from DNA through this enzyme.

Item number：BP-E11

Quality control

//cdn.myxypt.com/05aa6382/24/09/622afe66455a41fe4903260a18159fbca4734c34.png