HS Taq chemically modified enzyme

HS Taq is a hot-start Taq enzyme that is chemically modified to completely block the activity of Taq enzyme at room temperature. HS Taq can effectively inhibit non-specific amplification caused by non-specific annealing of primers or primer polymers under low-temperature conditions, thereby improving the specificity and sensitivity of PCR reactions. The "Time release" method can also be adopted. During the PCR cycle heating process, the enzyme activity is gradually released, which can further increase the specificity and sensitivity of low-copy template amplification. The main advantages of this enzyme in fluorescence PCR reactions are high sensitivity, high fluorescence value and high specificity.

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1．5 U/µL HS Taq 

2.10 ×HS Buffer (Mg²+ free) (Optional)

3.25mM MgCl₂ (optional)

The 10×HS Buffer (Mg²+ free) does not contain dNTPs and Mg²+. Please add DNTPS and MgCl₂ when preparing the reaction system.

1. Chemical modification, 95℃, hot start for 5 to 10 minutes.

2. The activity is gradually released and has strong specificity.

Endpoint method (E03), quantitative PCR.

2. Multiplex PCR, genotyping detection, and highly sensitive virus detection。

Quantitative gradient testing

2. Immediate amplification of comparative experiments

3. Single-enzyme accelerated experiment at 37℃