Bst 3.0

Bst DNA Polymerase 来源于 Bacillus stearothermophilus DNA PolymeraseⅠThrough genetic engineering technology, the 5'→3' DNA polymerase activity was retained, while the 5'→3' exonuclease activity was removed. It has strand displacement ability and can be used in DNA strand displacement reactions and LAMP (Loop-mediated isothermal amplification) amplification, etc. Bst 3.0 DNA polymerase has been genetically modified to have higher sensitivity. Compared with wild-type Bst DNA polymerase and large Bst fragments, this enzyme can effectively improve the anti-interference performance against inhibitors, amplification speed, yield, etc. Bst 3.0 DNA polymerase can utilize dUTP to construct the lamp anti-pollution system.

1．8 U/µL Bst 3.0 

2．10×LAMP Basic Buffer (Mg²+ free)（选配） 

3.250 mM MgSO₄ (optional)

*10×LAMP Basic Buffer (Mg²+ free) does not contain dNTP and Mg²+. Please add dNTPs and MgSO₄ when preparing the reaction system.

It can be used for LAMP.

2. Strong anti-inhibition, high impurity tolerance, and sample compatibilityhigh。

3. An anti-pollution system can be established at room temperature to effectively prevent aerosol contamination of the products.

1. Lamp isothermal amplification related technologies.

2. Establishment of a pollution prevention system.