Pfu DNA Polymerase Ⅱ

Pfu DNA Polymerase II is a high-fidelity enzyme that can catalyzePolymerization of deoxynucleotides dependent on DNA templates in the 5'→3' directionIt has 3'→5' exonuclease activity but no 5'→3' exonuclease activityIts amplification product is A flat terminal without any protruding A.
In addition, this enzymeBe capable of reading and amplifying templates containing uracil and hypoxanthine; theThe characteristics can be used to amplify bisulfite transformation, deamination or damageDNA, suitable for PCR anti-contamination systems (used together with dUTP and UDG).

1.  Pfu DNA Polymerase Ⅱ 5U/μL

2.  10×Pfu Buffer

High-fidelity enzyme, capable of catalyzing the polymerization of deoxynucleotides in the 5'→3' direction dependent on DNA templates, possesses 3'→5' exonuclease activity but lacks 5'→3' exonuclease activity.

2. Its amplification product is A flat terminal without any protruding A.

3. It can read and amplify templates containing uracil. This feature can be used to amplify bisulfite transformed, deaminated or damaged DNA, and is suitable for PCR anti-contamination systems.

High-fidelity PCR.

2. Methylation detection.