E.coli Total RNA Residue Detection Kit (PCR-Fluorescence Probe Method)

Escherichia coli, as a host for exogenous gene expression, has a clear genetic background, simple technical operation and simple culture conditions. It is the most widely used and successful expression system and is often highly cultivatedThe preferred system for effective expression. In the actual production process of viral vectors for gene therapy, large-scale GMP-grade plasmids are required for the transfection step, and the process also involves plasmid preparation"Ready. Multiple literatures have proposed that in addition to the host DNA residue present in the non-specific nucleases introduced by the process, plasmids also need to be strictly controlledE.coliResidual DNA of host cellsRNA and residual proteins.

This kit is based on the fluorescence quantitative PCR method. Primer probes are designed for the conserved sequences of the E.coli genome, combined with ready-to-use E.coli RNA quantitative reference materials. By integrating reverse transcription and fluorescence probe qPCR detection technologies, a rapid detection method has been established.highSensitive real-time fluorescence quantitative PCR kitwithFor quantitative detectionrawResidual in the productsbigTotal RNA of Enterobacter.

This kit, when used in conjunction with the Borui Biological DNA Removal Kit and Nucleic Acid Extraction or Purification Kit II, can detect total RNA residues of E.coli at the fg level in as fast as 2 hours.

This kit has undergone comprehensive methodological validation in accordance with relevant regulations, guidelines and other document requirements, and can provide performance validation reports.

Product item Number: BP-QN64-100

Product advantages：

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