mRNA Capping Rate Detection Pretreatment Kit (2.0)

This kit does not provide specific probes. Probes need to be designed based on the target sequence. The designed probe must consist of three parts (as shown in the figure) : 20 to 25 ribonucleotide sequences at the 3' end that are complementary and paired with the 5' end of the target sequence. To prevent the degradation of the probe, modified ribonucleotides can be used in this region. It is recommended to usewithMethoxy-modified nucleotides at position 2; Then comes a deoxyribonucleotide sequence complementary to the target sequence, and this region does not need to be modified; The final 3' end is modified with Biotin. A single probe synthesis within 10 days is sufficient to meet the required amount of probes for the specified number of times in this kit. For recommended suppliers of probe synthesis, please contact the technical support.

Item No. : BP-05-10

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