2×LAMP Premix (DyeⅠ)

LAMP (Loop-mediated isothermal amplification) loop-mediated isothermal amplification reaction is a nucleic acid amplification technology. This kit is a constant temperature amplification using the dye method (Eva Green) and can be used for qualitative detection of DNA in samples (such as viruses). Bst2.0 DNA Polymerase is derived from Bacillus stearothermophilus DNA Polymerase. Compared with wild-type Bst DNA polymerase and large fragments, this enzyme can effectively increase amplification speed, yield, etc. Bst2.0 DNA polymerase possesses 5 '→3' DNA polymerase activity and strong strand displacement ability, but lacks 5 '→3' exonuclease activity. It can be used for DNA strand displacement reactions and LAMP amplification, etc. Bst 2.0HS is a hot-start isothermal polymerase obtained by using reversible modification technology based on Bst 2.0 DNA polymerase. It can completely block the enzyme's activity at room temperature, establish reactions at room temperature, prevent non-specific amplification, and improve reaction efficiency. In addition, Bst 2.0HS DNA polymerase does not require a separate activation step.

This kit adopts LAMP technology. Six specific LAMP-specific primers are designed for the target sequence. The BST2.0HS isothermal amplifying enzyme and the specific primers are used to recognize the six independent regions of the target sequence. The double-stranded DNA generated by isothermal amplification binds to Eva Green and emits a fluorescence signal. The fluorescence signal is detected by an instrument to determine whether there is amplification.

Item Number:HW205 -R01

Freeze-dried item number: FHW1007-R01

Reagent composition

2×LAMP Premix Buffer Ⅱ、BST2.0 HS (8 U/µL)、50×Eva GreenEqual components.

 

Recommended application：

1.optimumwithFor the LAMP dye method detection of DNA samples, an instrument is required to detect fluorescence。

2.Establish the reaction at room temperature to prevent non-specific amplification and improve the reaction efficiency.

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