pFEN

pFEN is a thermally stable Flap endonuclease I that can catalyze the cleavage of 5 'flap DNA in branched double-stranded DNA substrates, generating a 5' phosphate terminal.

2.DNA ligases can link FEN1 products to generate double-stranded DNA.

In in vivo experiments, FEN1 is not only crucial for the maturation pathway of the Okazaki fragment but also participates in base excision repair.

Cut structure-specific DNA.