Plasmid Mini Kit

The Plasmid Mini Kit for plasmid extraction uses the alkaline lysis method to lyse cells. It utilizes a unique silica gel membrane adsorption column to specifically adsorb plasmid DNA under high-salt and low-pH conditions, removing proteins, RNA, genomes, and other impurities to obtain high-quality and high-purity plasmid DNA. Each adsorption column can adsorb up to 30 µg of plasmid DNA and is suitable for the efficient extraction of plasmid DNA from 1 to 5 mL of overnight cultured Escherichia coli. The yield and quality of plasmid extraction are related to factors such as the type of host bacteria and culture conditions, cell lysis, plasmid copy number, and plasmid stability. The plasmid DNA extracted by this kit can be directly applied to various routine molecular biology experiments such as enzyme digestion, ligation, PCR, sequencing and transformation.

1. Fast: Few steps, simple operation, and time-saving.
2. High efficiency: It can extract over 85% of the plasmid DNA from bacteria.

Plasmid extraction in small quantities is used in molecular biology experiments such as enzyme digestion, transformation, sequencing and PCR.

Plasmid Mini Kit was used to extract the same amount of bacteria under the same conditions as well-known competing products on the market, and they were eluted with 100 µL of elution solution. The above results show that
The yield of plasmids extracted by Baorui products is comparable to or slightly better than that of its competitors.
2. The bands are clear and bright, with no residual RNA.
3.Both the OD260/OD280 and OD260/OD230 ratios meet the standards of high-quality plasmids.