5×FastAmpli Premix UNG Ⅳ (NA) (Probe qPCR) (DG) (090803)

FastAmpli Premix-UNG Ⅳ (NA) (Probe qPCR) (DG) (090803) is a dedicated reagent developed specifically for freeze-drying processes and for real-time PCR qualitative and quantitative reactions using the probe method. It contains DNA polymerase that has been genetically modified and screened for rapid amplification, and can complete the entire PCR reaction within 30 minutes. This product has been optimized with a Buffer system and is suitable for multiple amplifications. This reagent contains enzymes specifically designed for freeze-drying systems and has been repeatedly optimized. This reagent uses a mixed enzyme of anti-inhibitory amplifying enzyme and UNG enzyme, as well as an optimized Buffer system containing dUTP. It not only enables good amplification of the target gene in samples containing inhibitors, but also effectively prevents false positive reactions caused by residual PCR products and aerosol contamination. This product is compatible with fluorescence quantitative PCR instruments from most manufacturers, such as Applied Biosystems, Eppendorf, Bio-Rad and Roche, etc. This reagent has a good freeze-dried form and product stability after freeze-drying. This product has been processed by a special technique and leaves no DNA residue. It can be used for the development of test reagents related to biopharmaceuticals.

1. 5×FastAmpli Premix-UNG Ⅳ (NA) (Mg^{2 +}free) (DG) (090803)
2. 50×MgCl₂
3.4 × Freeze-drying Protective Agent (optional)


1. The DNA polymerase must be a rapid amplifying enzyme with an amplification rate of no less than 1 KB per 10 seconds.

2. It is equipped with a complete anti-pollution system, including heat-sensitive UNG enzymes and dUTP. UNG enzyme can effectively digest PCR products containing dU at 50℃, and is completely inactivated during pre-denaturation at 95℃, without affecting the subsequent amplification efficiency.

3. Fully compatible and optimized support for the following rapid qPCR programs

(1). UNG digestion: 50℃, 2 minutes, 1 cycle;

(2). Pre-denaturation: 95℃, 30 seconds, 1 cycle;

(3). Amplification: Denaturation at 95 ° C for 1 to 3 seconds, annealing and extension at 60 ° C for 3 to 20 seconds, 40 to 45 cycles.

4. Specifically developed for freeze-drying processes, after adding the matching 4× freeze-drying protective agent in liquid state, it can maintain stable performance.

5. Through special processing techniques, it ensures no DNA residue and no activity of exogenous nucleases (endonucleases, exonucleases), guaranteeing the accuracy and reliability of the test results.

6. Compatible with the mainstream fluorescence quantitative PCR instruments on the market.

DNA virus detection related to humans and animals.

2. Amplification and typing detection of genomic DNA.

3. Rapid amplification detection.