DeoxyribonucleaseDeoxyribonuclease I, DNase I, is a non-specific endonuclease capable of digesting single-stranded or double-stranded DNA. It can water.Dissociate the phosphate diester bond to produce monodeoxynucleotides and oligodeoxynucleotides containing 5' -phosphate groups and 3'-OH groups. The activity of DNase I depends on Ca²+AndIt can be oxidized by divalent metal ions such as Mn²+,Mg²+,Zn²+Wait for activation. At Mg²+Under the existing conditions, DNase I randomly splays any site of double-stranded DNA; Mn²+ExistUnder certain conditions, DNase I can cleave DNA double strands at the same site to form flat ends or sticky ends with 1-2 nucleotides protruding.
This kit is based on the non-specific cleavage of single-stranded or double-stranded DNA by DNase I and is used in conjunction with the nucleic acid extraction or purification kit of Baorui Biology to establish a rapid method for preparing RNA samples without DNA for the subsequent detection of total RNA in biological products. RNA samples can be prepared in as fast as 1 hour.
Product item number:BP-QN65-100
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