2×Fast Direct RT Premix-UNG Ⅳ (Probe qRT-PCR)

Fast DirectRTPremix-UNG Ⅳ (Probe qRT-PCR) It is specifically designed to eliminate the nucleic acid extraction and purification steps of samples and directly conduct fluorescence quantification of target genes.TaqMThe an probe method is a dedicated reagent for RNA amplification detection, containing rapidly amplifying reverse transcriptases and DNA polymerases that have been genetically modified and screened, with an amplification rate of 20 to 40 The PCR reaction is completed within minutes. This product has a strong tolerance to inhibitors and can be used without nucleic acid extraction and purificationThroat swabAnticoagulated whole blood, plasma, serum and other samplesDirect RNA amplification detection.

This reagent is formulated with an optimized formulaThe qPCR-specific Buffer and UNG/dUTP anti-contamination system can effectively prevent false positive amplification caused by residual PCR products and aerosol contamination.

1. 10×Fast Direct RTase/UNG Mix Ⅳ

2. 2×Fast Direct RT Premix Buffer Ⅳ (dUTP)

It has excellent tolerance to inhibitors from sample sources such as plasma, swabs, and tissue homogenates. The direct sample addition ratio is ≤5% for whole blood and ≤30% for plasma.

2. It is suitable for rapid fluorescent quantitative RT PCR without extraction, and the detection can be completed within 40 minutes at the fastest.

Detection of RNA viruses related to humans and animals.

2. Direct and rapid amplification detection without extraction.

Immediate amplification comparison experiment