5×FastAmpli RT Premix-UNG (Probe qRT-PCR)

FastAmpli RT Premix-UNG (Probe qRT-PCR) is a dedicated reagent for fluorescence quantitative (TaqMan probe method) RNA amplification detection. It contains reverse transcriptase and DNA polymerase that have been genetically modified and screened for rapid amplification, and can complete the PCR reaction within 20 to 40 minutes. This product has strong inhibition tolerance, higher reverse transcription and PCR amplification efficiency, and is suitable for high-sensitivity amplification with low-concentration RNA templates. It can obtain good standard curves within a wide quantitative range and accurately perform quantification.

This reagent uses a mixed enzyme of anti-inhibitory amplifying enzyme and UNG enzyme, as well as an optimized Buffer system containing dUTP. It not only enables good amplification of the target gene in samples containing inhibitors, but also effectively prevents false positive reactions caused by residual PCR products and aerosol contamination.

This product is compatible with fluorescence quantitative PCR instruments from most manufacturers, such as Applied Biosystems, Eppendorf, Bio-Rad and Roche, etc.

1. 10×FastAmpli RTase/UNG Mix

2. 5×FastAmpli RT Premix Buffer (dUTP) (Mg^{2 +}free) (DG)

3. 25×MgCl₂

The amplification rate is no less than 1kb/10s, suitable for rapid fluorescence quantitative PCR of pure RNA, and the detection can be completed within 30 minutes at the fastest.

2. It has excellent specificity and sensitivity.

Detection of RNA viruses related to humans and animals.

2. Amplification and typing detection of genomic RNA.

3. Rapid amplification detection.

1. Evaluation of M5144 Acceleration stability

Experimental conditions

Experimental results


Summary: After being placed at 37℃ and accelerated for 7 days and 14 days, the amplification performance of M5144 reagent was consistent with that of the unaccelerated reagent and could remain stable.

2. Freeze-thaw stability evaluation of M5144

Experimental conditions

Experimental results

Summary: After 5, 10, 15, and 20 freeze-thaw cycles, the amplification performance of the M5144 premix reagent remains consistent with that of the unthawed reagent and can maintain stability.

3. Rapid amplification Evaluation of M5144

Experimental conditions

Experimental results


Summary: M5144 reagent can stably detect HCV-MLN templates under rapid amplification procedures and is suitable for rapid amplification.

4. Amplification efficiency test of M5144

Experimental conditions

Experimental results

Summary: The M5144 reagent exhibits an excellent linear relationship over a wide range of template concentrations, with good amplification efficiency and reliable repeatability of quantitative results.

5. Verification of freeze-drying accelerated stability of FM5144 (55℃)

Experimental conditions

Experimental results

Test method: Place the freeze-dried samples at 55℃ and accelerate for 30 days. Then, use liquid reagents as the control to conduct comparative tests.

Summary: PFM5144-O25 was accelerated at 55℃ for 30 days, and its amplification performance was consistent with that of liquid reagents, remaining stable.