Varicoxvirus capping enzyme

In eukaryotes, the 5' end of mature mrnas formed by post-transcriptional modifications all have a special structure, namely the m7GPPPN structure, also known as the methylguanosine cap. hatThe childAs a necessary structure for the initiation of mRNA translation, it not only protects mRNA from being cut by RNase, prolongs its half-life, but also enhances the stability of mRNA during translation, splicing, and export from the nucleus. The Vaccinia Capping Enzyme (VCE) independently developed by Baorui Biotechnology integrates mRNA triphosphatase, guanosine acyltransferase andThe birdThe necessary activities of capping enzymes such as purine methyltransferase, in S-adenosylmethionine (SAM) as a methyl donor andThe birdIn the presence of glycoside triphosphate (GTP), it can directly and efficiently add the m7G cap structure to the 5' end of mRNA in the correct direction to produce RNA with a Cap0 cap structure, achieving a maximum capping rate of 100% in a single reaction. When combined with the 2'-O-Methyltransferase (2OM) (BP-E06) from Baorui Biotechnology, RNA with a Cap1 cap structure can be obtained.

Item Number: BP-E05

Quality control

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