Dioxymethyltransferase

2'-O-Methyltransferase (2OM) is a methyltransferase encoded by the DNA of the bovine pox virus expressed in recombinant E.coli. It is a methyltransferase that further transforms the Cap0 cap structure into the Cap1 cap structure. This enzyme uses S-Adenosylmethionine (SAM) as a methyl donor and adds a methyl group at the 2'-O site of the first nucleotide adjacent to the cap structure (Cap0) at the 5' end of RNA to form mRNA with a Cap1 structure. It can further reduce the immunogenicity of mRNA itself and increase the expression level of the encoded protein after transfection.

The solution is clear and transparent, with no visible foreign substances.
There is no activity of exonuclease or endonuclease.
Protein purity ≥95%.
No DNase or RNase activity.